淡紫拟青霉NH-PL-03菌株对甘薯茎线虫的毒力效应

Toxic Effect of Paecilomyces lilacinus (Thom.) Samson str. NH-PL-03 Culture Filtrate on Ditylenchus destrnctor Thorne

研究了培养基、温度、通气量对淡紫拟青霉NH-PL-03菌株毒力的影响,结果表明:总体上,菌液对甘薯茎线虫的毒力随发酵时间的增长而上升;孟加拉红、PDA和察培克有利于毒力产物分泌,其中察培克培养基的毒力较高,对甘薯茎线虫最高校正死亡率为43.4%;在15℃～30℃范围内,温度升高,毒力也随之上升,30℃条件下毒力最高,培养132h菌液对甘薯茎线虫的较正死亡率达到48.8%;较低的通气量(转速60r/min、装瓶量40%)有助于毒力提升,对甘薯茎线虫的最高校正致死率分别达到47.5%、57.1%.

Growth of Paecilomyces lilacinus on different medium, temperature, and aerating condition and its efficacy against juveniles of Ditylenchus destrnctor Thorne had been investigated. The result showed that as a whole the NH-PL-03 filtrate has significant toxic effects on Ditylenchus destrnctor Thorne, the revised mortality of treated juveniles increase along with culture continues. Culturing medium rich in C、N source and mineral ion(such as Czapek)expressed higher toxic effects(43.4%). 30℃ of culture temperature and lower aerating condition(60r/min of rotating speed and 40% of loading capacity)exhibited higher nematicidal activity, in this culturing condition, the max. revised mortality of Ditylenchus destrnctor was 48.8%,47.1% and 57.1% respectively.