营养启动剂诱导类炭疽芽胞发芽的研究

Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant

目的 观察营养启动剂诱导类炭疽芽胞杆菌芽胞发芽的生物效应。方法 通过热激活和营养启动剂诱导类炭疽芽胞发芽;分光光度计测量芽胞悬液在600 nm的吸光度值(A),计算不同条件下的发芽率;透射电镜观察发芽过程中芽胞超微结构的变化。结果 在 37℃ pH 7.9的条件下,6 mmol/L次黄嘌呤核苷营养诱导剂使类炭疽芽胞发芽率为68.0%;在 30℃ pH 8.9的条件下,70 mmol/L L 丙氨酸营养诱导剂使类炭疽芽胞发芽率为74.5%;两者的混合营养诱导剂可明显提高发芽率,达到85.6%;透射显微电镜下观察,见类炭疽芽胞出现外衣及皮质层破裂、降解,核心暴露。结论 在人工调整的适宜环境下,以次黄嘌呤核苷和L 丙氨酸为主要成分的营养启动剂可快速启动类炭疽芽胞发芽。

Objective To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant. Methods Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores. Results The rate of germination effects were (68.0) % under (6 mmol/L) inosine at 37℃, pH (7.9); (74.5)% under (70 mmol/L) (L-alanine) at 30℃, pH (8.9); and (85.6)% under (6 mmol/L) inosine and (70 mmol/L) (L-alanine) at 37℃, pH (8.2). Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed. Conclusion Under suitable environment, the nutrient germinant with inosine and (L-alanine) might be helpful for germinating the bacillus anthracoides spores.