转抗菌肽基因辣椒株系的青枯病抗性鉴定及系统选育

Evaluation of Resistance to Bacterial Wilt and Systemic Breeding in Cecropin-GM Capsicum

利用通过农杆菌介导技术获得的T0代转抗菌肽基因辣椒植株为试材,对其自交株系世代群体连续进行抗青枯病鉴定筛选、分子生物学检测和系统选育,首次获得了具有抗青枯病能力的转抗菌肽基因辣椒稳定株系。同时,对其主要果实性状、青枯病抗性进行的对比试验结果表明,转抗菌肽辣椒株系除抗青枯病能力明显提高外,果实性状基本不变。上述结果显示,外源目的基因主要特性的遗传是稳定的、表达是忠实的,从实践上验证了转抗菌肽基因工程操作的实用性。

Using T0 generation of cecropin-GM capsicum that was obtained by Agrobacterium-mediated method as testing material, the resistance to bacterial wilt of its inbred lines generations were continuously screened and identifyed by molecular biology method and systemic breeding. As a result, a cecropin-GM capsicum with the resistance to bacterial wilt were obtained for the first time. Moreover, the resistance to bacterical wilt and fruit trait were compared between the transformants and their non-transgenic lines respectively. The results showed there were no significant difference in fruit traits, but the resistance to bacterial wilt was raised markedly in the cecropin-GM capsicum. All these results indicated that cecropin-GM gene was stablely expressed and inherited in offspring of transformants, and that cecropin-GM gene engineering technique was feasible to bacterial wilt control.