酶法提取牛腱胶原的研究

Study on extracting collagen of bovine tendon with enzyme

以牛肌腱为原料,通过酶法来提取Ⅰ型胶原,找出其最佳工艺条件,并对所提取的胶原进行了性能表征。酶法水解的最佳条件:进口胃蛋白酶用量的质量分数1 % ,pH值2 .5及4℃条件下水解作用3 0h。研究结果提示:酶解法是比较理想的提取方法,不仅提取产率高,而且提取的胶原样品的分子质量高(1 0万Dal以上)且分布窄,可初步判定其基本维持了天然胶原的三股螺旋结构,适合作为生物医用材料的原料。

TypeⅠcollagen was prepared from bovine tendon by enzymatic method treating with pepsin.The optimum project was arrived at. TypeⅠcollagen prepared was characterized. The various physico-chemical properties, such as molecular weight, IR spectra ,DSC and amino acid component of bovine tendon collagen were measured.As a result,enzymatic hydrolysis is the premium extracting methods, which is both high extraction ratio, high molecular weight above 100 000 Dalton and origioal triple helix structure.The collagen prepared fits well for biomedical use.