壳聚糖对肉仔鸡肠道菌群的影响

Effect of chitosan on intestinal microflora in broilers

试验选择1日龄、体重42.8±2.6g的AA雄性肉仔鸡294只,随机分成7个日粮处理组,每个处理6个重复,每个重复7只鸡,试验期为42d。7种日粮是在基础日粮中分别添加0.00%(对照组)、0.02%、0.05%、0.10%、0.30%、0.50%壳聚糖和50mg/kg金霉素配制而成。结果表明:日粮各处理组对肉仔鸡盲肠内容物中总需氧菌无显著影响;大肠杆菌在14和42日龄时也未显示出显著的组间差异,而在28日龄时组间差异趋于显著(P=0.121),添加壳聚糖的各处理组均不同程度地低于对照组,其中以0.05%壳聚糖组最低;乳酸菌在14日龄时无显著的组间差异,在28日龄时,添加壳聚糖的各处理组相对于对照组均有不同程度的升高趋势,尤以0.05%壳聚糖组最高,而金霉素组则极显著低于0.05%～0.50%壳聚糖组(P=0.008),在42日龄时,乳酸菌的组间无显著差异(P=0.201),但变化趋势与28日龄时类似。可见,添加适宜剂量的壳聚糖可在一定程度上改善肉仔鸡的肠道微生态环境。

The experiment was conducted to study the effects of dietary chitosan(0.00 %,0.02 %,0.05 %,0.10 %,0.30 % and 0.5 %,respectively)and chlortetracycline(50 mg/kg)on cecal microfloral concentration in broilers.The results showed that dietary treatments had no influence on total aerobe concentration in caecum ingesta of broilers in each period.On day 14 and 42,there was no difference among dietary treatments,but on day 28,0.05 % dietary chitosan suppressed proliferation of E.coli compared with the control(P = 0.121).On day 14,the concentration of Lactobacill had no difference among treatments.But on day 28,the concentration of Lactobacill in chitosan treatments presented a rising tendency compared with the control.Especially,0.05 % ～ 0.50 % dietary chitosan improved significantly proliferation of Lactobacill(P = 0.008)compared with chlortetracyline,and 0.05 % chitosan had the best effect.On day 42,there was a similar tendency to that on day 28.The results suggested that an appropriate dose of dietary chitosan could improve intestinal microflora in broilers to a certain extent.