转录组与蛋白质组比较研究进展

Progress in The Comparison of Transcriptome and Proteome

转录组和蛋白质组比较研究发现,总体而言其间的相关性不高. 根据数据的类型不同可以将现有的研究分为4类:单点比较、两点差异比较、多点时序比较和多点非时序比较. 对其差异原因的研究和分析表明:除了由实验系统及数据类型不同导致的差异外,转录后蛋白质合成各步骤所受到的限制,以及在此过程中的分子调控也对其有重要的影响;而且不同基因,不同组织和细胞在不同状态下可能也会有差异. 因此,结合转录组和蛋白质组的表达谱研究倾向于利用蛋白质组和转录组研究的差异和互补性,同时对生物体特定状态下的基因和蛋白质表达水平进行全方位度量,以获得表达谱的全景图,并挖掘受到转录后调控的基因.

Moderate correlations between transcriptome and proteome were found in most studies. According to different data types, current studies could be divided into four categories. They are comparison on single point, comparison between two differential points, comparison among multiple time-sequence points and comparison among multiple non-time-sequence points. In addition to the experimental error and the different datasets, the post-transcription restriction and regulatory would affect the correlation very much. Different results might be got for the different genes, cells, organs, organisms and even the different developmental phases. Because of the differentia and complementary between them, parallel studies of transcriptome and proteome trended to be performed, which could get the panorama screen of gene expression and discover the genes regulated in post-transcription.