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人类新型β3半乳糖基转移酶基因β3GalT7的克隆和鉴定 被引量：3

Cloning and Characterization of a Novel Human β3-Galactosyltransferase Gene β3GALT7
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摘要 从人肺cDNA文库中克隆到人类β3-半乳糖基转移酶家族中的一个新成员β3GalT7(AY277592,EC2.4.1.-),并对其进行了鉴定. 该基因定位在人类染色体19q13.2. 它包含一个1 191 bp的开放阅读框(ORF). 编码的蛋白质包括一个信号肽和一个半乳糖基转移酶结构域,分子质量和等电点分别为43.3 ku和8.37. 从原核表达中获得的融合蛋白的分子质量和预测相符. RNA印迹杂交显示β3GalT7在肺、喉和回肠组织中高表达,而在舌、乳房、子宫、睾丸等组织中表达较低. 此外半定量RT-PCR显示β3GalT7在人类不同的肿瘤细胞中转录水平有明显差异. A novel human β3-galactosyltransferase gene β3GALT7 was isolated from human lung cDNA library and characterized. β3GALT7 is mapped to chromosome 19q13.2 by browsing the UCSC genomic database. It contains an ORF with length of 1 191 bp, encoding a protein with a signal peptide sequence and galactosyl-T domain, and its molecular mass and isoelectric point are predicted to be 43.3 ku and 8.37 respectively. The molecular mass of recombinant β3GALT7 protein expressed in prokaryote was coincide with the predicted. Northern blot showed that β3GALT7 was highly expressed in lung, throat and ileum, while the expression level was low in tongue, breast, uteri, testis. In addition, it was also demonstrated that β3GALT7 is differentially transcribed in human tumor cell lines.
作者 周嘉梁 黄超群 吴士良 孙其昌 雷伟 金美芳 姜智 沈宏杰
机构地区 苏州大学医学院基础医学系生物化学与分子生物学教研室 苏州大学生化工程研究所
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2005年第2期140-146,共7页 Progress In Biochemistry and Biophysics
基金 苏州大学医学发展基金资助项目.~~
关键词 β3GalT7 克隆 RNA印迹 肿瘤细胞株 galactosyltransferase, clone, Northern blot, tumor cell line
分类号 Q53 [生物学—生物化学]
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参考文献10

  • 1Sasaki K, Sasaki E, Kawashima K, et al. Japanese Patent, 0618759 A 940705. 1994-07-05.
  • 2Kolbinger F, Streiff M B, Katopodis A G. Cloning of a human UDP-galactose:2 -acetamido-2-deoxy-D-glucose 3-galactosyltransferase catalyzing the formation of type 1 chains. J Biol Chem, 1998, 273 (1): 433～440.
  • 3Amado K, Almeida R, Cameiro F, et al. A family of human β3-galactosyltransferases. J Biol Chem, 1998, 273 (21): 12770～12778.
  • 4Isshiki S, Togayachi A, Kudo T, et al. Cloning, expression, and characterization of a novel UDP-galactose: β-N-acetylglucosamine β1,3-galactosyltransferase (β3Gal-T5) responsible for synthesis of type 1 chain colorectal pancreatic epithelia and tumor cells derived thereform. J Biol Chem, 1999, 274 (18): 12499～12507.
  • 5Bai X M, Zhou D P, Brown J R, et al. Biosythesis of the linkage region of glycosaminoglycans. J Biol Chem, 2001, 276 (51):48189～48195.
  • 6Krauss T, Walther K, Lakoma C, et al. Circulating endothelial cell adhesion molecules as diagnostic markers for the early identification of pregnant woman at risk for development of preeclampsia. Am J Obstet Gynecol, 1997, 177 (2): 443 ～ 449.
  • 7Amado M, Almeida R, Carneiro F, et al. A family of human β3-galactosyltransferase: characterisation of four members of a UDPgalactose:β-N-acetylglucosamine/β-N-acetylgalactosamineβ1,3-galactosyltransferase family. J Biol Chem, 1998, 273 (21):12770～ 12778.
  • 8Hennett T, Dinter A, Kuhnert P, et al. Genomic cloning and expression of three murine UDP-galactose: β-N-acetylglucosamine β 1,3-galactosyltransferase gene. J Biol Chem, 1998, 273 (1): 58～65.
  • 9Miyaki H, Fukumoto S, Okada M, et al. Expression cloning of rat cDNA encoding UDP-galactose G (D2) β1,3-galactosyltransferase that determines the expression of G(D1b)/G (M1)/G (A1). J Biol Chem, 1997, 272 (40): 24794～24799.
  • 10Zhou D, Berger E G, Hennett T. Molecular cloning of a human UDP-galactose: GlcNAcβ1,3GalNAc β1,3 -galactosyltransferase gene encoding an O-linked core 3-elongation enzyme. Eur J Biochem, 1999, 263 (2): 571 ～576.

同被引文献28

  • 1代智,刘银坤.糖基转移酶与肝癌发生[J].国外医学（肿瘤学分册）,2005,32(2):144-146. 被引量：5
  • 2Huang C, Zhou J, Wu S, et al. Cloning and tissue distributrion of a the human β3GalT7 gene, a member of the β1,3-Glycosyhransferase family [ J]. Glycoconj J, 2004,21(5): 267 -273.
  • 3Bai X,Zhou D, Brown JR, et al. Biosynthesis of the linkage region of glycosaminoglycans : cloning and activity of galactosyhransferase Ⅱ, the sixth member of the beta 1,3-galactosyltransferase family ( beta 3 GalT6 ) [ J ]. J Biol Chem,2001,276(51) : 48189-48195.
  • 4Ishida H, Togayachi A, Sakai T, et al. A novel beta1, 3-N-acetylglucosaminyhransferase ( beta3Gn-T8 ), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer[ J]. FEBS Lett, 2005, 579 (1):71-78.
  • 5Hassan H, Reis CA, Bennett EP, et al. The lectin domain of UDP-N-acetyl-D-gatactosamine: polypeptide N- acetyl galactosaminyltransferase-T4 directs its glycopep tide specificities [ J ]. J Biol Chem, 2000,275 (49) : 38197 - 38205.
  • 6Kjellen L, Lindahl U. Proteoglycans: structures and inter- actions [ J ]. Annu Rev Biochem, 1991, 60 : 443 - 475.
  • 7Huang C, Zhou J, Wu S, et al. Cloning and tissue distri- bution of the human B3GALT7 gene, a member of the be- ta1 ,3-Glycosyltransferase family [ J ]. Glycoconj J, 2004, 21(5) : 267 -273.
  • 8Hiroyasu Ishida, Akira Togayachi, Tokiko Sakai, et al. A novel β1,3 -N -acetylglucosaminyltransferase ( β3Gn - TS), which synthesizes poly-N-acetyllactosamine, is dra- matically upregulated in colon cancer[ J]. FEBS Letters, 2005,579(1) : 71 -78.
  • 9J·萨姆布鲁克,D·W·拉塞尔.黄培堂,等译著.分子克隆试验指南[M].北京:科学出版社,2002:1713-1726.
  • 10Kim YJ, Varki A. Perspectives on the significance of al- tered glycosylation of glycoproteins in cancer [ J ]. Glyco- conj J, 1997, 14(5) : 569 -576.

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生物化学与生物物理进展
2005年 第2期

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