哮喘患者CD4^+T细胞对单核/巨噬细胞抗原递呈功能的影响

Effects of CD4^+T lymphocytes on processing mononuclear phagocyte in patients with asthma

目的 :探讨支气管哮喘患者 CD4 + T细胞对单核 /巨噬细胞抗原递呈功能的影响。方法 :哮喘患者 2 0例 ,健康对照 2 2例 ,分别取静脉血 5 m L ,并分离单核 /巨噬细胞、 CD4 + T细胞和 B细胞。每份血样分成 4组 :MΦ递呈抗原组、 CD4 + T细胞参与 MΦ递呈抗原组、 CD4 + T细胞体外活化后对 MΦ递呈抗原影响组及自然状态下CD4 + T细胞对 MΦ细胞递呈抗原影响组。各组用 CTL L2 P抗原刺激 18h后 ,洗去刺激原 ,与自体 B细胞共同孵育 10 d,吸取上清液 ,测定特异性 Ig M、 Ig E、 Ig G含量。结果 :哮喘患者 CD4 + T细胞与单核 /巨噬细胞共同培养递呈抗原时 ,产生特异性 Ig M (A4 90 nm) (0 .0 33± 0 .0 31)低于健康人 (0 .0 98± 0 .0 6 2 ) (P<0 .0 5 ) ;产生特异性 Ig G(A4 90 nm) (0 .0 80± 0 .0 35 )明显高于健康人 (0 .0 5 5± 0 .0 2 4 ) (P<0 .0 5 ) ;产生特异性 Ig E (A4 90 nm) (0 .15 4±0 .119)的调节作用与健康人 (0 .186± 0 .10 1)差异无显著性 (P>0 .0 5 ) ;在无抗原刺激的自然状态下 ,哮喘患者CD4 + T细胞与递呈抗原单核 /巨噬细胞共同培养 ,也能产生高水平的特异性 Ig G(哮喘组 :0 .0 75± 0 .0 18,对照组 :0 .0 5 7± 0 .0 18;P<0 .0 5 )。结论 :哮喘患者 CD4 + T细胞对单核 /巨噬细胞递呈?

Objective To study the effects of CD4^+ T lymphocytes on processing mononuclear phagocyte in patients with asthma. Methods The mononuclear phagocytes and CD4^+ T lymphocytes were isolated from peripheral blood in 20 patients with asthma and 22 healthy donors, respectively. Every sample was divided into four groups:MΦA^+,(MΦCD4^+)A^+,MΦ^+CD4^+A^+,and MΦA^+CD4^+. All groups were stimulated with CTLL 2P antigen for 18 hours and CTLL 2P antigen were washed away, then they were cultured with B lymphocytes together, the levels of secreted CTLL 2P-specific IgM, IgG, and IgE in the supernatant were measured after 10 days. Results When CD4^+ T lymphocytes were cultured with mononuclear phagocytes together, the level of CTLL 2 P-specific IgM of patients with asthma (A 490 nm)(0.033±0.031) was lower than that of healthy donors(0.098±0.062)(P<0.05); the level of CTLL 2 P-specific IgG of patients with asthma (A 490 nm)(0.080±0.035)was significantly higher than that of healthy donors(0.055±0.024)(P<0.05);the same result was obstained (P<0.05), when primate CD4^+ T lymphocytes were cultured with antigen processing mononuclear phagocytes (patients:0.075±0.018,healthy donors:0.057±0.018). The CTLL 2 P-specific IgE level of patients with asthma(0.154±0.119) was as same as healthy donors (0.186±0.101)(P>0.05). Conclusion The CD4^+ T lymphocytes of patients with asthma have different regulatory functions on immunolglobulins from antigen processing mononuclear phagocytes.