CA125及CA19-9抗原在子宫内膜异位症组织中的表达及意义

Expressions of CA125 and CA19-9 in tissues of patients with endometriosis and their significances

目的 :探讨 CA12 5及 CA19- 9抗原在子宫内膜异位症子宫内膜组织中的表达意义及其与血清值之间的相关关系。方法 :应用免疫组织化学 S- P法观察 2 0例子宫内膜异位症患者异位子宫内膜 (卵巢 )及其在位子宫内膜 CA12 5及 CA19- 9的蛋白质表达 ;应用磁性分离酶联免疫法测定 2 0例子宫内膜内异症患者血清 CA12 5及CA19- 9的含量。结果 :CA12 5及 CA19- 9蛋白质主要表达于异位子宫内膜腺体上皮细胞。CA12 5蛋白质在异位子宫内膜及在位子宫内膜的阳性表达率分别为 90 .0 %及 2 5 .0 % ;CA19- 9蛋白质在异位子宫内膜及在位子宫内膜的阳性表达率分别为 85 .0 %及 10 .0 %。 6例 、 期子宫内膜异位症患者血清 CA19- 9、CA12 5值 (U· m L- 1 )及阳性率分别为 (2 9.6 4± 10 .2 4 )、 (2 5 .85± 9.4 5 )及 16 .7%、 33.3%。 14例 、 期子宫内膜异位症患者血清 CA19- 9、CA12 5值 (U· m L- 1 )及阳性率分别为 (46 .5 4± 14 .2 9)、 (5 8.0 2± 2 0 .11)及 4 2 .8%、 71.4 %。异位内膜 CA19- 9免疫组化染色阳性率为 85 .0 % (17/ 2 0 ) ,与血清中 CA19- 9测定值之间呈正相关关系 (rs=0 .36 ,P<0 .0 5 )。结论 :CA12 5及 CA19- 9蛋白质在子宫内膜异位症患者的异位子宫内膜呈高表达 。

Objective To investigate the expressions of CA125 and CA19-9 in endometriosis and the relationship with the serum values of CA125 and CA19-9. Methods Serum CA125 and CA19-9 levels were measured by using the magnetic separated enzyme linked immunosorbent assay respectively in 20 patients with sendometriosis. Forty paraffin samples of endometriosis(20 ectopic endometria and 20 endometria)were examined by immunohistochemical staining method to detect the expressions of CA125 and CA19-9. Results CA125 and CA19-9 were mainly expressed in the cytoplasm of glandular epithelial cells. The positive expression rates of CA125 in ectopic endometrium and endometrium were 90.0% and 25.0%, respectively;The expression rates of CA19-9 in ectopic endometrium and endometrium were 85.0% and 10.0%, respectively. The mean serum values of CA19-9 and CA125(U·mL~ -1)in 6 patients with stage Ⅰ and Ⅱ endometriosis were (29.64±10.24) and (25.85±9.45),respectively; the positive rates were 16.7% and 33.3%,respectively. The mean serum value of CA19-9 and CA125 (U·mL~ -1) in 14 patients with stage Ⅲ and Ⅳ endometriosis were (46.54±14.29 ) and (58.02±20.11), respectively;the positive rates were 42.8% and 71.4%, respectively. The expression of CA19-9 in ectopic endometrium was correlated to the serum value of CA19-9 (r s=0.36,P<0.05), but not CA125 (r s=0.12,P>0.05). Conclusion The CA125 and CA19-9 proteins express highly in ectopic endometrium, and express lowerly in endometrium. The main resource of serum CA19-9 in patients with endometriosis is ectopic endometrium.