HHV6感染与风湿性和类风湿性关节炎相关性的研究

Study on relationships between HHV 6 infection and rheumatic arthritis and rheumatoid arthritis

目的 探讨人疱疹病毒 6型(humanherpesvirus6,HHV6)感染在风湿性关节炎 (rheumaticarthritis,RhA)和类风湿性关节炎(rheumatoidarthritis,RA)病因学中的作用。方法 用套式PCR(nested PCR)检测 40例RhA、62例RA患者和 138例健康献血员外周血白细胞(PBWC)和血浆中HHV6DNA。结果 RhA组和RA组PBWC中HHV6DNA阳性率与正常对照组比较均无显著性差异(P>0. 05);部分RhA和RA患者血浆中可检出HHV6DNA,而对照组血浆中HHV6DNA阴性。结论 部分RhA、RA患者血浆中存在HHV6DNA,提示这些患者可能存在HHV6活动性感染。

Objective To investigate the etiology of rheumatic arthritis and rheumatoid arthritis (RA) in which human herpesvirus 6(HHV 6)infection may play a role.Methods HHV 6 DNA in plasma and peripheral blood mononuclear cells from 40 rheumatic arthritis patients,62 RA patients and 138 matched healthy adult donors was detected by nested PCR.Results There was no significant difference for the positive rate of HHV 6 DNA in the peripheral blood mononuclear cells between patients and controls group ( P > 0.05).By nested PCR HHV 6 DNA were positive in some of plasma samples from rheumatic arthritis patients or RA patients,while none of the controls was HHV 6 DNA positive.Conclusions These results indicated that there are higher positive rate of HHV 6 DNA in the peripheral blood mononuclear cells of rheumatic arthritis patients and RA patients. Some of the patients were HHV 6 DNA positive in plasma,while none of the controls was HHV 6 DNA positive.It suggested that active infection of HHV 6 may be related to the pathogenesis of rheumatic arthritis and RA.