重组人红细胞生成素对大鼠脊髓损伤后神经细胞凋亡的保护作用

Protective effect of recombinant-Human Erythropoietin on neuronal apoptosis after spinal cord injury in rats

目的 观察大鼠脊髓损伤(SCI)后损伤段脊髓神经细胞凋亡机制和相关因子的表达,以及重组人红细胞生成素(rHu-EPO)对其的影响。方法 30只SD大鼠分为4组,建立SCI模型,观察rHu EPO对大鼠神经功能的影响;免疫组化法检测损伤脊髓神经细胞凋亡因子(Bcl 2、Bax、Fas)的表达;TUNEL标记凋亡细胞;比较各组间差别。结果 治疗组的神经功能较损伤组提高(P<0.05);损伤组的凋亡阳性细胞多于治疗组;治疗组Bcl 2阳性表达细胞多于损伤组(P<0.05)。结论 凋亡是 SCI后神经细胞死亡的一种重要方式;rHu-EPO能抑制脊髓神经细胞凋亡,对损伤脊髓的神经功能有保护作用。

ObjectiveTo observe the expression of apoptosis factor, the mechanism of neuronal apoptosis and profective effect of recombinant Human Erythropoietin (rHu EPO) after spinal cord injury (SCI) in SD rats.Methods30 SD rats were divided into four groups including control group, SCI group, treatment groups A and B. The animal SCI model was established with Allen's method. The changes of nerve functions of rats of 4 groups were observed before and after treating with rHu EPO. The expressions of apoptosis factors (Bcl 2, Bax, Fas) were tested with immunocytochemistry technique, and apoptosis neurones were labeled with TUNEL dyeing.ResultsThe grade of nerve function was improved distinctly in treatment groups, but group B was better than group A. The number of the positive cells for Fas and Bax in SCI group was more than that in treatment groups ( P <0 05), and group A was more than group B. The number of Bcl 2 positive cells in the treatment groups was greater than that in SCI group ( P <0 05).ConclusionApoptosis is a important death mode of neuron after SCI. rHu EPO can distinctly improve the comeback of the nerve function and protect the nerve tissue. [