大肠癌患者外周血CK20表达及p53基因突变的研究

Study of Cytokeratin 20 Expression and p53 Gene Mutation in Peripheral Blood of Patients with Colorectal Carcinoma

目的 建立高效、快速的外周血中细胞角蛋白 2 0 (Cytokeratin 2 0 ,CK2 0 )表达的检测方法 ,探讨大肠癌患者外周血中CK2 0的表达与微转移的关系 ,研究大肠癌与p5 3基因突变的关系。方法 运用荧光监测技术结合RT -PCR ,检测 3 6例大肠癌患者术前和术后不同时间外周血中的CK2 0的表达。用SSCP技术对大肠癌患者进行p5 3基因的突变检测。结果 3 6例患者的外周血中检出CK2 0表达的有 2 7例 ,阳性率为 75 % ,手术后 2 4h患者外周血中检出CK2 0表达的有 3 0例 ,阳性率为 83 3 % ,术后早期化疗结束后仅 13例为阳性 ,阳性率为 3 6%。 3 6例患者的p5 3基因的SSCP突变检测中未检测到突变。结论 运用荧光PCR技术检测外周血中CK2 0的表达可以监测癌症微转移的发生 ,简便快速 ,检出率高。

Objective To set up an efficient and rapid method for detecting CK20 expression in peripheral blood of patients with colorectal carcinoma, and explore the relationship between CK20 expression and micrometastasis of colorectal carcinoma. Methods CK20 mRNA expression in peripheral blood of 36 patients with colorectal carcinomas was detected at various time points before and after operation by fluorescent RT-PCR. The mutation of p53 gene was also detected using SSCP. Results The positive rate of CK20 mRNA expression in peripheral blood before and 24 hours after operation was 75%(27/36) and 83.3%(30/36), respectively. After the short-term chemotherapy, the positive rate of CK20 mRNA expression was 36%(13/36). No mutation of p53 gene was found in those patients. Conclusion Fluorescent RT-PCR for detecting CK20 mRNA expression in peripheral blood was simple and efficacious. It was useful to monitor micrometastasis of colorectal carcinoma.