分米波对大鼠再生神经NGF mRNA表达的影响

Effect of decimeter wave on the expression of NGF mRNA in regenerated nerve

目的研究分米波对周围神经损伤后再生神经中神经生长因子(NGF)表达的影响。方法选用60只SD大鼠,随机分为实验组和对照组各30只,均于右侧坐骨神经中段切除5mm神经组织,硅胶管桥接神经缺损,形成神经再生室,实验组术后进行局部分米波辐射。于术后1,2,4和8周取材,行大体和光镜观察,并进行免疫组化检测及RTPCR定量分析。结果实验组NGF阳性染色颗粒及积分光密度值(IOD)明显高于对照组。实验组各时间点再生神经纤维中NGFmRNA的含量均明显高于对照组(P<0.01),同一神经纤维中NGFmRNA表达量近侧明显高于远侧(P<0.05)。实验组再生神经纤维中NGFmRNA于术后1周即有表达,术后2~4周达到高峰,术后8周后开始下降。结论分米波能增加神经纤维中NGFmRNA的表达,促进周围神经再生。

Objective To investigate the effect of decimeter wave on the expression of nerve growth factor(NGF) in regenerated nerve after peripheral nerve injury. Methods Sciatic nerve in the middle right legs of 60 SD rats was resected for 5mm, and the two cut ends were bridged by a silicone tube which served as a nerve regeneration chamber. The experimental group was treated by decimeter wave after operation. The bridged nerves were sampled at the 1st,2nd,4th,8th week, respectively after operation and the samples were observed by light microscope, histochemistry and reverse transcription polymerase chain reaction(RT-PCR). Results In decimeter wave sides, the expression of immunologic reaction to NGF and integration optical density were higher than those of the control sides. In regenerated nerves, the level of NGF mRNA in decimeter wave sides was higher than that in the control sides ^(P<0.01) . In regenerated nerves, the level of NGF mRNA in the proximal stump was higher than that in distal stump (P<0.05). In decimeter wave sides, NGF mRNA appeared at the 1st week after operation. During the treatment period, the level of NGF mRNA was gradually increased and peaked at 2~4 weeks after operation and then began to decrease at the 8th week after operation. Conclusion Decimeter wave could increase the expression of NGF mRNA in regenerated nerves and promote regeneration of peripheral nerve.