番鸭精清中几种化学成分的分析

A STUDY ON THE CHEMICAL COMPOMENTS OF MUSCOVEYDUCK SEMINAL PLASMA

本文用离子选择电极法测定番鸭精清中几种无机离子的含量；用氨基酸自动分析仪测定精清中游离氨基酸的含量；此外，还测定了番鸭精清中葡萄糖、果糖等有机物质的含量以及几种酶的活力。结果表明，番鸭精清中Ｎａ￣＋、Ｋ￣＋、Ｃａ￣＋、Ｍｇ￣（２＋）和Ｃｌ￣－的浓度分别为：１２６．２±１０．８、１０．０７±２．５８、３．５３±０．７９、３．９６±０．９３和１２４．３±１０．６ｍｍｏｌ／Ｌ；在所有被测定的游离氨基酸中，谷氨酸含量（９３．１８±１４．６６ｍｇ／１００ｍｌ）较高，占被测定的１７种游离氨基酸总量的６０％以上。精清中葡萄糖含量（１１３．３±２３．５ｍｇ／１００ｍｌ）大大高于果糖含量（１．０５±０．５１ｍｇ／１００ｍｌ）。甘油三酯、总胆固醇、尿酸及尿素氮的含量分别为１１．４±４．８、１７．５±５．３、２４．５３±１０．５３及１．８７±０．５９ｍｇ／１００ｍｌ。精清中酸性磷酸酶活力（６２．１±３５．８金氏单位）大大高于碱性磷酸酶活力（５．６±２．８金氏单位），谷草转氨酶及谷丙转氨酶的活力分别为１７２±３６及２５６±１２０金氏单位。乳酸脱氢酶、苹果酸脱氢酶及６－磷酸葡萄糖脱氢酶的活力之比约为６７：２４：１。

This study was undertaken to determine some of the cheinical components of musco-vey duck (Cairina moschata) seminal Plasma. The result showed that the contents ofNa+, K+, Ca(++), Mg(++) and Cl- were 126.2±10.8, 10.07±2.58, 3.53±0.79,3.96±0.93, and 124.3±10.6mmol/L respectively. Among the 17 free amino acids measured,the amount of glutamic acid (93.18±14.66mg/100ml) was the highest; The amounts ofglucose, fructose, glyceride, total cholesterol, uric acid and urea were 113/3±23.5,1.05±0.51, 11.4±4.8, 17.5±5.3, 24.53±10.53 and 1.87±0.59 mg/100 ml respec-tively. The activity of acid phosphatase (62.1±35.8 King unit) was much higher thanthat of alkali phosphatase (5.6±2.8 King unit).There was no aPParent difference bet-ween the activities of glutamic-pyruvic transaminase (256±120 King unit) and glutamic-oxaloacetic transaminase (172±36 King unit).The ratio among the activities of lacticdehydrogenase, malate dehydrogenase and glucose-6-phosphate dehydrogenase was 67:2.4:1 approximately.