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巨噬细胞集落刺激因子对喉鳞状细胞癌喉癌细胞株的作用 被引量：1

The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line
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摘要 目的:了解和探讨巨噬细胞集落刺激因子(MCSF)及其受体对喉鳞状细胞癌细胞株(Hep2)细胞系的作用。方法:使用氚标记胸腺嘧啶核苷(3HTDR)掺入法和MTT法测试MCSF作用于Hep2后细胞的增殖状况,并测试加入巨噬细胞集落刺激因子受体(MC-SFR)抗体后细胞生长情况。ELISA法测试M-CSF作用Hep2细胞后上清中的MCSF的浓度变化。结果:Hep2细胞培养后较培养前的上清中的MCSF的浓度升高。加入的M-CSF浓度越大,细胞增殖越差,加入MCSFR抗体浓度越大细胞增殖越好。结论:MCSF对喉癌细胞Hep2细胞的增殖具有抑制作用。 Objective:To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.Method:Cell proliferation was assayed by MTT analysis and thymidine incorporationx,after Hep-2 cell line culturing with M-CSF and M-CSFR.M-CSF concentration of surpernatant measured by a ELISA technique. Immunohistochemistry was performed on Hep-2 cell line and laryngeal carcinoma tissue.Result:The concentration of M-CSF increased after cell culturing.M-CSF can reduced the Hep-2 cell count.The action can be blocked by Anti-M-CSFR.Conclusion:Our observations support the hypothesis that M-CSF may inhibit the growth and differentiation in Hep-2 cell line.
作者 葛平江 张宝泉 张建民 高志强 孔庆利 何维
机构地区 广东省人民医院耳鼻咽喉头颈外科 中国医学科学院北京协和医学院北京协和医院耳鼻咽喉科 中国医学科学院中国北京协和医学院基础研究所免疫室
出处 《临床耳鼻咽喉科杂志》 CSCD 北大核心 2005年第6期265-268,共4页 Journal of Clinical Otorhinolaryngology
基金 国家973重点课题基金资助项目(No2001CB5100009)
关键词 喉肿瘤 癌.鳞状细胞 巨噬细胞集落刺激因子 增殖 HEP-2细胞系 Laryngeal neoplasms Carcinoma,squamous cell Macrophage colony stimulating factor Proliferation Hep-2
分类号 R739.65 [医药卫生—肿瘤]
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参考文献13

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二级参考文献8

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同被引文献7

  • 1刘磊玉,唐圣松.巨噬细胞集落刺激因子与肿瘤[J].国外医学（肿瘤学分册）,2005,32(7):499-503. 被引量：6
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  • 7王昕,张倩,宋玉华,吴克复.恶性细胞中巨噬细胞集落刺激因子及其受体的异源表达[J].中国实验血液学杂志,1998,6(1):24-28. 被引量：1

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临床耳鼻咽喉科杂志
2005年 第6期

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