注意缺陷障碍儿童血清兴奋性和抑制性氨基酸的检测及分析

Excitatory and inhibitory amino acids in serum of children with attention deficit hyperactivity disorder.

目的 通过检测注意缺陷障碍(ADHD)儿童血中兴奋/抑制性氨基酸代谢水平,探求它们与ADHD的关系及与心理行为的相关性。方法 采用反向高效液相色谱法,测定2 0 0 3年8月在上海第二军医大学附属长征医院随机抽取的5 8例注意缺陷障碍儿童(ADHD组:其中注意缺陷为主型2 2例;多动 冲动为主型4例;混合型32例)和30例健康对照儿童血清兴奋性氨基酸中的谷氨酸(Glu)、天冬氨酸(Asp)和抑制性氨基酸甘氨酸(Gly)的浓度并进行比较。结果 (1)ADHD组儿童血清兴奋性氨基酸Glu、Asp浓度分别为(16 9. 13±2 6 . 6 2 ) μmol/L和(2 9 .19±2 . 5 6 ) μmol/L ,显著低于对照组(P <0 .0 1) ,而抑制性氨基酸Gly浓度为(489. 78±96 . 2 6 ) μmol/L ,显著高于对照组(P <0 .0 5 )。(2 )不同类型ADHD儿童之间血清兴奋性和抑制性氨基酸浓度未见有统计学意义上的差异。(3)兴奋性和抑制性氨基酸浓度和DSM Ⅳ父母版所得各项分数之间无相关性。结论 ADHD儿童血清中存在兴奋/抑制性氨基酸的失调,此种失调可能参与了ADHD的病理生理过程。

Objective To understand the change of excitatory and inhibitory amino acid in serum of children with attention deficit hyperactivity disorder and its relationship with ADHD and behavioral psychology.Methods With reverse-phase and high performance liquid chromatography(HPLC),the levels of glutamic acid(Glu)、aspartic acid(Asp) and glycine(Gly) were determined in serum of 58 children with attention deficit hyperactivity disorder (22 attention deficit type、4 hyperactivity type and 32 mixture type) aged 7～14 years,and 30 age-and gender-matched healthy controls.Results (1)The contents of EAA,Glu[(169.13±26.62)μmol/L]、Asp[(29.19±2.56)μmol/L]in ADHD children,were obviously lower than that of controls ( P <0.01);the contents of IAA,Gly[(489.78±96.26)μmol/L] in ADHD children were obviously higher than that of controls( P <0.05).(2)There was no significant difference in level of amino acids between different types of ADHD children.(3)There was no relativity between the levels of excitatory and inhibitory amino acid and all kinds of marks of ADHD RS-Ⅳ-Parent:Inv.Conclusion It is definite that the numerical change of excitatory and inhibitory amino acids in serum takes place in children with attention deficit hyperactivity disorder.It may participate in the pathology of attention deficit hyperactivity disorder.