积雪草苷对瘢痕成纤维细胞增殖与磷酸化Smad2和Smad7表达的影响(英文)

Effect of asiaticoside on proliferation of scar fibroblasts and expression of phosphorylated Smad2 and Smad7

背景:转化生长因子-β(TGF-β)是病理性瘢痕形成的重要因素。Smad蛋白家族是近年来发现的TGF-β受体下游信号蛋白。积雪草苷可抑制成纤维细胞增殖及胶原的合成,使瘢痕内的TGF-β表达减少。目的:研究积雪草苷对瘢痕成纤维细胞增殖与磷酸化Smad2和Smad7的作用及机制。设计:以细胞为研究对象,对照、观察性研究。单位:一所大学医院烧伤整形科。对象:实验于2002-04/2003-03在中山大学外科实验室完成。标本取自因增生瘢痕住院进行整形手术的患者6例,男3例,女3例,年龄1～35岁。增生性瘢痕成纤维细胞由本科实验室经原代培养后传代获得。干预:研究分为实验组和对照组,将积雪草苷作用于成纤维细胞为实验组,对照组不加积雪草苷,观察用药前后各指标的改变。主要观察指标:①积雪草苷对磷酸化Smad2和Smad7的影响。②积雪草苷对细胞周期和凋亡的影响。结果:积雪草苷可抑制瘢痕的成纤维细胞从S期进入M期,减少成纤维细胞中磷酸化Smad2的含量,两组差异无显著性意义(t=1.53,P=0.08),细胞中Smad7的含量实验组为(50.80±22.40)%,对照组(32.18±17.84)%,两组的差异有显著性意义(t=2.17,P=0.024)。结论:积雪草苷抑制瘢痕可通过Smad通路发挥作用。

BACKGROUND:Transforming growth factora2 |? (TGFa2 |? ) is an essential factor for pathological scar formation.Smad protein group is the signal protein of lower reaches of TGFa2 |? receptor.Asiaticoside can inhibit proliferation of fibroblasts and synthesis of collagen to reduce TGFa2 |? expression in the scar.OBJECTIVE:To study the mechanism of asiaticoside on proliferation of scar fibroblasts and phosphorylated Smad2 and Smad7.DESIGN:Controlled study with observation,in which cell was taken as the object.SETTING:Department of burn and plastic surgery of a hospital affiliated to a university.PARTICIPANTS:The experiment was performed in Surgical Laboratory in Sun Yata2 sen University from April 2002 to March 2003.The specimens were selected from 6 inpatients receiving plastic operation due to hyperplasic scar including 3 male and 3 female cases aged varied from 1 to 35 years.The hyperplasic scar fibroblasts were obtained generated from original culture in laboratory of surgical department. INTERVENTIONS:In the research,the experiment group and the control group were divided.In the experiment group,asiaticoside was applied on fibroblasts;in the control group,asiaticoside was not prescribed. The changes of every index were observed before and after medication.MAIN OUTCOME MEASURES:￠ù Effect of asiaticoside on expression of phosphorylated Smad2 and Smad7;￠ú Effect of asiaticoside on cell cycle and apoptosis.RESULTS:Asiaticoside inhibited scar fibroblasts entering M phrase from S phrase and reduced the content of phosphorylated Smad2 in fibroblasts,which did not present significant difference in two groups (t=1.53, P=0.08).The content of Smad7 in the cells was (50.80?à 22.40)￡$ in the experiment group and (32.18?à 17.84)￡$ in the control group,which indicated significant difference (t=2.17, P=0.024).CONCLUSION:Asiaticoside inhibits scar formation by Smad passage.