实验性脑出血大鼠血肿周围不同时间点组织形态学的动态变化

Dynamically histomorphological changes in perihematoma at different time points following experimental intracerebral hemorrhage in rats

目的:研究大鼠实验性脑出血各病变期间炎症、水肿、神经元改变及胶质增生等因素的动态变化特征。方法:实验于2003-06/2004-06在河北医科大学第二医院神经内科实验室完成。用24只SD大鼠,实验组将自体血注入右尾状核,假手术组做对照。取血肿周围脑组织进行光学显微镜观察。结果:血肿周围脑组织内部分神经元变性坏死;出血后6h即可见少数单个散在炎性细胞浸润,48h达高峰,以中性粒细胞为主;72h后可见血肿周围及血肿内胶质细胞、血管增生;7d时血肿明显缩小,胶质细胞及血管增生明显。结论:脑出血后血肿周围炎性细胞浸润,神经元变性、坏死,胶质细胞和血管增生可能是脑水肿加重和病情加重的主要原因之一。

AIM:To dynamically observe the pathological changes of inflammation,edema,neuronal change gliosis,etc.in rats with experimental intracerebral hemorrhage(ICH). METHODS:The experiment was finished in the Department of Neurology,Second Hospital of Hebei Medical University from June 2003 to June 2004.Twenty- four Sprague- Dawley rats were enrolled in the study. Autologous blood was injected into the right caudate nucleus in experiment group.Sham operation group served as the control group.Perihermatoma tissues were collected for observation under light microscope. RESULTS:Some neurons in the perihematoma region displayed necrosis.Several scattered inflammatory cells were observed in the perihematoma region at 6 hours after ICH and the inflammatory infiltration characterized by neutrophils reached to the top at 48 hours.At 72 hours after ICH gliosis and capillary hyperplasia were observed in and around hematoma.The area of hematoma reduced markedly,accompanied by distinct glial and capillary hyperplasia at 7 days after ICH. CONCLUSION:Inflammatory infiltration,neuronal degeneration,neuron necrosis,glial and capillary hyperplasia in perihematoma of experimental ICH rats may be one of the severe causes of the severity of brain edema and disease.#