自体骨髓细胞移植对大鼠局灶性脑缺血区新生血管形成的作用(英文)

Effect of autologous bone marrow cell transplantation on angiogenesis in focal cerebral ischemia in rats

背景:骨髓细胞移植是一种简单而有效的促进新生血管生长的治疗手段。同样新生血管的形成和血液循环的再建立,对于脑缺血区域受损而未死亡神经元的修复以及为植入的组织与细胞的成活和分化提供必要的环境条件均具有重要意义。而自体骨髓细胞移植是否会促进脑缺血区新生血管的形成进行血运重建目前尚不清楚。目的:探讨经颈动脉移植自体骨髓细胞对脑缺血区新生血管形成的影响。设计:以实验动物为研究对象,随机对照的验证性研究。单位:一所大学医院的神经外科和泌尿外科研究所。材料:实验于2002-09/2003-04在江西医学院第二附属医院神经外科实验室,江西医学院泌尿外科研究所完成。选择雄性SD大鼠10只,体质量250～300g,清洁级。干预:复制局灶性脑缺血大鼠模型,经颈动脉注入自体骨髓细胞。动物随机分为实验组5只,经颈动脉注入自体骨髓细胞,对照组5只注入生理盐水。通过免疫组织化学染色方法进行微血管计数,观察脑缺血区血管增生状况。主要观察指标:微血管密度。F8因子免疫组化染色。结果:自体骨髓细胞移植后大鼠在皮质缺血区的微血管密度为(159.15±40.4)血管数/mm2,明显较对照组大鼠(81.70±32.18)血管数/mm2多,差异有显著性意义(P<0.05)。骨髓细胞移植组皮质缺血区可见很多散在的单个内皮细胞。

BACKGROUND:Bone marrow cell transplantation is a simple and effective treatment to promote angiogenesis.The neovascularization and reestablishment of blood circulation is crucial to the repair of injured but still living neurons as well as to the survival and differentiation of the implanted tissues and cells.However,it is not clearly known whether autologous bone marrow cells implantation could promote neovascularization and facilitate reestablishment of circulation in ischemic regions of the brain. OBJECTIVE:To investigate the effect of autologous bone marrow cell transplantation via the carotid artery on angiogenesis in the ischemic regions of the brain.DESIGN:A randomized controlled experiment based on experimental animals.SETTING:Department of neurosurgery and institute of urinary surgery in a university hospital.MATERIALS:The experiment was conducted in the Laboratory of the Department of Neurosurgery,Second Affiliated Hospital,and the Institute of Urinary Surgery,Jiangxi Medical College during the period from September 2002 to April 2003,using 10 specific pathogen- free male SD rats weighing 250- 300 g. INTERVENTIONS:Rat models of focal cerebral ischemia model were established and randomized equally into two groups.The rats in the transplantation group were injected with autologous bone marrow cells via the carotid artery and those in the control group received normal saline injection.The angiogenesis in the ischemic regions of the brain was observed with immunohistochemical staining for microvessel counting. MAIN OUTCOME MEASURES:The density of microvessels. Immunohistochemical staining for F8.RESULTS: Greater density of the microvessels was found in the ischemic regions after bone marrow cell transplantation than in the control group[(159.15± 40.4)/mm2 vs(81.70± 32.18)/mm2,P< 0.05].Numerous endothelial cells were found scattered in the ischemic cortex of the transplantation group,but scarcely in the control group. CONCLUSION:Autologous bone marrow cell transplantation via the carotid artery can promote angiogenesis in the ischemic regions of the brain.