摘要
背景:一氧化氮合酶(nitricoxidesynthase,NOS)广泛分布于中枢神经系统并调节神经活动,但鲜见在中枢神经脊上水平有关NOS参与内脏神经传入信息传递的报道。目的:探查三叉神经脊束间质核(interstitialnucleusoftrigeminaltract,INV)内内脏传入终末与向臂旁核(parabrachialnucleus,PBN)投射的NOS阳性神经元之间的联系。设计:以实验动物为研究对象,验证性对比观察。单位:一所军医大学解剖学教研室。材料:选择成年SD雄性大鼠10只进行动物试验。干预:分别给PBN及舌咽和迷走神经内注射四甲基罗丹明(tetramethylrhodamine-dextran,TMR),生物素化葡聚糖胺(biotinylateddextran-amine,BDA)并进行NOS免疫组织化学反应,结合激光扫描共聚焦显微镜观察。主要观察指标:观察逆行TMR标记细胞、NOS阳性细胞以及跨神经节BDA标记的传入终末在INV内的重叠分布和三者的相互关系。结果:逆行标记细胞主要位于注射侧的INV,大多属20μm以下的中、小型细胞。NOS阳性细胞与TMR逆行标记细胞分布区域重叠。计数显示:NOS/TMR双标记细胞分别占NOS阳性细胞总数的55%(17/31)和TMR逆行标记细胞总数的34%(17/49)。跨神经节注射BDA标记的内脏神经初级传入终末点状膨体贴近双标记细胞胞体,呈紧密接触状。
BACKGROUND:Nitric oxide synthase(NOS) is extensively distributed in the central nervous system and modulates neuronal activities.However,there are few reports about NOS's involvement in nociceptive information transmission at the supraspinal level. OBJECTIVE:To investigate the relation between the primary afferent visceral terminals and the NOS- containing neurons which project from the interstitial nucleus of spinal trigeminal tract(INV) to the parabrachial nucleus(PBN). DESIGN:A controlled experimental observation based on the experimental animals. SETTING:Department of anatomy in a military medical university of Chinese PLA.MATERIALS:The experiment was conducted in the Department of Anatomy,Fourth Military Medical University of Chinese PLA,from January to December 2002.Ten adult male SD rats were selected in this experiment. INTERVENTIONS:Tetramethyl rhodamine- dextran(TMR) and biotinylated dextran- amine(BDA) were injected into the PBN and vago- glossopharyngeal nerves respectively combining with the immunohistochemical method for NOS and the observation with laser confocal scanning microscope. MAIN OUTCOME MEASUREES:Overlapping of TMR retrogradely labeled neurons,NOS positive labeled neurons and transganglionic tracing labeled terminals,and their relationship in INV. RESULTS:After injection of TMR into the PBN,the retrogradely labeled neurons,mostly small and medium- sized(below 20 μ m),were found in the INV ipsilateral to the injection side.The distribution of the NOS positive neurons overlapped with retrogradely labeled neurons in INV.The proportion of the number of double- labeled neurons with NOS/TMR was 55% (17/31) and 34% (17/49) in the whole population of the NOS positive neurons and retrogradely neurons, respectively.Some transganglionic tracing labeled terminals after BDA injection had close contact with the double- labeled neurons.CONCLUSION:The visceral nociceptive pathway exists from INV to PBN and NO may act as the transmitter or neural signal molecule in the transmission and regulation of visceral nociceptive process.#
出处
《中国临床康复》
CSCD
北大核心
2005年第9期215-217,i006,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(90209025)~~