骨髓间充质干细胞通过分泌TGF-β1抑制T细胞的增殖

Mesenchymal stem cells inhibit T-lymphocyte proliferation through secreting TGF-β1

目的研究是否MSCs通过分泌TGFβ1抑制PHA刺激的T淋巴细胞的增殖。方法应用酶联免疫吸附试验测定MSCs上清中转化生长因子TGFβ1的分泌水平,应用抗TGFβ1抗体中和MSCs上清中的TGFβ1后,用3H掺入法检测在不同培养条件下PHA刺激T淋巴细胞增殖的能力,以及应用Elispot试剂盒检测T淋巴细胞IFNγ的分泌水平。结果MSCs上清减少活化的T淋巴细胞IFNγ的分泌,抑制PHA刺激的T淋巴细胞的增殖能力,且它的这种抑制作用能够被抗TGFβ1抗体部分逆转。结论MSCs通过分泌TGFβ1抑制PHA刺激的T淋巴细胞的增殖。

Objective:To study if MSCs inhibit T-lymphocyte proliferation through secretion of transforming growth factor-beta (TGF-β1).Methods:The level of TGF-β1 was evaluated by enzyme linked immunosorbant assay (ELISA);after the TGF-β1 was neutralized by its correspondig mAb,the ability of T lymphocyte-proliferation was measured by ~~ 3H -Thydimine incorporation;and the level of interferon-gamma (IFN-γ) was measured by Eli-spot kit.Results:MSCs reduced the level of IFN-γ secreted by activated T lymphocytes;inhibited the ability of T-lymphocyte proliferation and such an effect could be reversed partly by anti-TGF-β1 antibody.Conclusion:MSCs inhibit T-lymphocyte proliferation through secreting TGF-β1. [