摘要
目的探讨白细胞介素5(interleukin-5,IL-5)和核因子κB(nuclear factor kappaB,NF-κB)在变应性鼻炎动物模型骨髓中表达的意义。方法采用6-8周雄性SD大鼠,随机分成实验组10只和对照组10只,以卵清蛋白致敏激发制成变应性鼻炎模型,免疫组化染色检测骨髓中IL-5和NF-κB的表达。结果大鼠变应性鼻炎模型骨髓涂片中IL-5阳性细胞和NF-κB比例都显著高于对照组(P<0.05),从形态上判断IL-5阳性细胞和NF-κB阳性细胞是嗜酸性粒细胞,IL-5阳性细胞比例和NF-κB阳性细胞比例呈显著正相关(r=0.79,P<0.01)。结论大鼠变应性鼻炎动物模型骨髓原位嗜酸性粒细胞能产生IL-5和NF-κB,可能调节骨髓中嗜酸性粒细胞的增殖和分化。
OBJECTIVE To elucidate the mechanism of bone marrow response regulated by interleukin-5 (IL-5) and nuclear factor kappa B(NF- κ B) in the model of allergic rhinitis. METHODS Twenty SD rats were randomly divided into allergic rhinitis group(AR group) and control group(Con group).The expression of IL-5 and NF- κ B were detected by immunohistochemistry.RESULTS The ratio of IL-5 positive cells to white cells in bone marrow smears of AR group was significantly higher than that of Con group ( P< 0.05 ).The ratio of NF- κ B positive cells to white cells in bone marrow smears of AR group was significantly higher than that of Con group (P< 0.05 ).Most of the IL-5 positive cells and NF-κ B positive cells were eosinophils morphologyically. The ratio of NF- κ B positive cells to white cells was significantly positively correlated with the ratio of IL-5 positive cells to white cells in bone marrow smears of AR group ( r= 0.79,P < 0.01). CONCLUSION Eosinophils express IL-5 and NF-κ B located in bone marrow of the rat model of allergic rhinitis and could regulate the proliferation and differentiation of eosinophils in bone marrow.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2005年第2期103-105,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
国家十五科技攻关计划(2003BA712 A11--17)国家自然科学基金资助项目(30400495)
