猪急性胰腺炎模型的建立及CT特点

Establishment of acute pancreatitis in pig model and its CT features

目的 探讨建立家猪急性胰腺炎模型的方法,观察其 CT表现。方法 10 只家猪采用牛磺胆酸钠和胰蛋白酶经主胰管注射法,5只家猪采用牛磺胆酸钠及无水酒精被膜下直接注射法分别制作急性胰腺炎模型,行 CT平扫及薄层动态增强扫描,24h后处死动物观察胰腺病理组织学变化。结果 牛磺胆酸钠和胰蛋白酶经主胰管注射后, CT显示胰腺体积明显肿胀,胰腺实质内密度不均,胰周大片液性阴影,肾前筋膜增厚,增强扫描胰腺不均匀强化,可见多个局灶性低密度无强化区;胰腺被膜下及实质内直接注射药物仅造成一过性胰腺炎症。结论 采用牛磺胆酸钠和胰蛋白酶经主胰管注射法可诱导出理想的猪急性胰腺炎。

Objective To discuss the way to establish acute pancreatitis in pig and observe its CT features. Methods Ten acute pancreatitis models were established by injecting sodium taurocholate and trypsin into the main pancreatic duct, and 5 acute pancreatitis models were established by injecting sodium taurocholate and alcohol into the pancreas capsule. CT plain scanning and thin-slice dynamic enhanced scanning were undergone respectively. Pigs were sacrificed to observe pancreatic pathological finding 24 hours later. Results After sodium taurocholate and trypsin was injected into the main pancreatic duct, pancreas volume was tumefacient, parenchyma density was unequal, peripancreatic fluid shadow was seen, and kidney anterior fascia thickened in CT plain scanning . Pancreas was unequally enhanced in dynamic enhanced scanning and many focal low density areas were found. Transient pancreatitis appeared after sodium taurocholate and alcohol was injected into the pancreas capsule. Conclusion Pig acute pancreatitis model could be established satisfactorily using sodium taurocholate and trypsin injected into the main pancreatic duct.