转移抑制基因BRMS1对卵巢癌细胞侵袭能力的影响

Effect of Metastasis Suppressor Gene BRMS1 on the Invasion Ability of Ovarian Cancer Cell

目的研究转移抑制基因BRMS1对高转移性人卵巢上皮癌细胞(HO-8910PM)侵袭能力的影响。方法脂质体介导将重组真核表达质粒pcDNA3.0-BRMS1转染HO-8910PM细胞;RT-PCR检测转染前后细胞中BRMS1mRNA的表达;Boyden小室法检测细胞侵袭能力。结果与未转染细胞(HO-8910PM)和pcDNA30空质粒转染细胞(HO-8910PM-vect)相比,BRMS1基因转染细胞(BRMS1.c2)侵袭穿透Matrigel基质膜的细胞数降低70.6%(P<0.001)。结论BRMS1基因能抑制卵巢癌细胞HO-8910PM的侵袭能力,为进行卵巢癌基因治疗提供了实验依据。

Objective To investigate the effect of metastasis suppressor gene BRMSl on the invasion ability of a highly metastatic human ovarian cancer cell line ( HO-8910PM). Methods The recombinant eukaryotic expression vector ( pcDNA3. 0-BRMS1 ) was transfected into the human ovarian carcinoma cell line ( HO-8910PM ) by lipofectamine. The expression of BRMSl in cells before and after transfection was detected by RT-PCR. The motility of the BRMSl transfected cells was observed in Boyden chamber test. Results Compared to untransfected parental cells (HO-8910PM) and pcDNA3.0 vect-only transfected cells (HO-8910PM-vect) ,BRMSl transfectants (BRMS1. c2) appeared that its number of invasion through Matrigel filter was significantly decreased ( P < 0. 001 ). Conclusion Metastasis suppreor gene -BRMSl significantly suppresses ovarian cancer cells invasion. This study provides experimental data for gene therapy of ovarian carcinoma with BRMSl.