摘要
目的 探讨Smad_4和Smad_7在人脑胶质瘤中的表达及意义。方法 采用免疫组化方法,对临床经过石蜡包埋的30例胶质瘤和8例正常脑组织标本进行定位和定性检测。结果 Smad4和 Smad7蛋白在胶质瘤中有不同程度的表达,在正常脑组织、低、高级别胶质瘤中 Smad4 的阳性表达分别为44.80±16.19,25.81±9.48,6.73±3.71,P<0.05。Smad7 的阳性表达分别为6.69±3.86,18.22±7.84,41.95±17.27,P<0.05。差别均有显著意义。且Smad4与Smad7的表达水平呈负相关,r=-0.82,P<0.01。结论 Smad_4 随胶质瘤的病理分级增高阳性表达降低,对胶质瘤的进展有抑制作用。Smad_7 则相反,可能参与了胶质瘤的恶性进展。
Objective To investigate the expression of Smad 4 and Smad 7 proteins, which are intracellular signaling molecules of transforming growth factor-β family in human glioma.Methods The expression levels of Smad 4 and Smad 7 in 30 cases of human glioma and 8 cases of normal brain tissue specimen were detected by immunohistochemical technics.Results In normal brain tissue, groupⅠ,Ⅱ and Ⅲ, Ⅳ, the positive expression levels of Smad 4 was 44.80 ± 16.19 , 25.81 ± 9.48 , 6.73 ± 3.71 respectively, and Smad 7 was 6.69 ± 3.86 , 18.22 ± 7.84 , 41.95 ± 17.27 respectively. The difference were significant ( P < 0.05 ). The expression of Smad 4 was lower in glioma than normal tissue. However, the expression of Smad 7 was positively correlated with the tumor grade. Smad 4 and Smad 7 were negative correlation( r = -0.82 ). Conclusion These finding suggested that Smad 7 play an important role during the malignant behaviors of glioma. Smad 4 could be a good tumor suppressor gene of glioma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2005年第3期132-134,F002,共4页
Cancer Research on Prevention and Treatment
