脂质体-c-raf-1反义寡核苷酸转染对人卵巢癌裸鼠皮下移植瘤形成的作用

Effects of Liposome-c-raf-1 Antisense Oligodeoxynucleotides Transfection on the Human Ovarian Cancer Transplanted Subcutaneously in Nude Mice

目的 探讨经脂质体一硫代磷酸化修饰的c raf 1反义寡脱氧核苷酸(ASODN)作用后的卵巢上皮癌细胞株SKOV3在裸鼠皮下的成瘤能力。方法 利用脂质体将经硫代磷酸化修饰的寡核苷酸导入SKOV3细胞,然后将这种被转染的细胞接种于裸鼠皮下,观察肿瘤体积的变化,并计算抑瘤率。结果 经脂质体c raf 1 ASODN作用后的卵巢上皮癌细胞在裸鼠皮下成瘤能力降低,最大抑瘤率达35.3%(P<0.05)。首次出现肉眼可见肿瘤的平均时间延长为8.8天(P <0.05)。结论 c raf 1 癌基因的反义调控能降低卵巢上皮癌细胞的成瘤能力,抑制肿瘤生长,在卵巢上皮癌的基因治疗中有一定作用。

Objective To investigate the effects of liposome c raf 1 antisense phosphorothioate oligodeoxynucleotides (c raf 1 ASODN) on the human ovarian epithelial cancer transplanted subcutaneously in nude mice.Methods Human ovarian epithelial cancer cell lines SKOV 3 were treated with liposome c raf 1 ASODN, then transplanted subcutaneously in nude mice. The Changes of tumor volume were observed and the tumor growth inhibitory rate was calculated.Results The tumorigenic ability of the treated cell lines was reduced, the maximum tumor growth inhibitory rate was 35.3% ( P < 0.05 ). The first time that tumor can be detected was increased up to 8.8 days ( P < 0.05 ).Conclusion The results suggested that c raf 1 ASODN transfection can inhibit the tumorigenesis of ovarian epithelial cancer cell lines in nude mice, it may be a useful gene therapy for the ovarian epithelial carcinoma.