APOA基因多态性与颅内动脉瘤的相关性

Correlation between APOA Gene Polymorphism and Intracranial Aneurysm

目的研究APOA基因多态性与颅内动脉瘤发病的相关性。方法选择58例经全脑动脉造影证实为颅内动脉瘤的患者及健康对照组58例,采集血浆,提取淋巴细胞基因组DNA,采用多聚合酶链反应(PCR)结合非变性聚丙烯酰胺凝胶电泳(PAGE)检测APOA5'调控区五核苷酸TTTTA重复序列(PNR)的基因多态性,运用统计学方法分析基因与疾病的相关性。结果两组人群共检出8种APOAPNR基因型和6种等位基因。动脉瘤组PNR-5等位基因频率和PNR5/8基因型频率显著高于健康对照组(P<0.05)。PNR-9等位基因频率和PNR8/9基因型频率显著低于健康对照组(P<0.05)。结论APOAPNR(TTTTA)拷贝数为5的等位基因可能与颅内动脉瘤发生呈正相关。

Objective To study the correlation between a pentanucleotide repeats(PNR)polymorph-ism of APOA and the genesis of intracranial aneurysm. Methods Fifty-eight patients with intracranial aneurysms diagnosed by angiography and 58 healthy controls were enrolled in our study. The gene polymorphism of APOA PNR were detected by polymerase chain reaction(PCR)and non-denatured polyacrylamide gels electropherosis(PAGE). Results Eight kinds of gene types and 6 kinds of alleles were found in these two groups. There were two sites of sequence variance in the 5'control region of APOA gene, which were significantly different between the patients and controls. Conclusion Correlation may exist between intracranial aneurysm and APOA gene.