渗透压与pH值对常温保存山羊精液的影响

THE EFFECT OF OSMOTIC PRESSURE AND pH OF DILUTER ON ROOM TEMPERATURE SEMEN OF BUCK

本文研究了山羊精液在高倍稀释（１：３０），常温（１３—１５℃）保存条件下，稀释液渗透压与ｐＨ值对精子存活时间的影响。试验选用两种稀释液，渗透压调为较高的３４１和３３９渗压毫克分子浓度与较低的２６３和２８０渗压毫克分子浓度，ｐＨ值分别调为６．５和７．０。结果表明：在渗透压类同的条件下，ｐＨ为６．５，精子总存活时数比ｐＨ为７．０时长（ｐ＜０．０１）。生存指数高（Ｐ＜０．０１）。在ｐＨ值相同的条件下，较高渗透压的稀释液保存效果好。渗透压与ｐＨ值对精子共同作用下，在ｐＨ６．５时，不论渗透压较高或较低，精子活力维持在０．３以上的时数为３０小时左右，而ｐＨ７．０时只有１６小时左右。较低渗透压使精子顶体完整率下降轻快（Ｐ＜０．０１）。

The semen samples of bucks were collected to study the effect of osmotic pressure and pH of diluter onsperm survive under high dilution(1:30)and storage in room temperature. The experiment was designed using twodiluters with two levels of osmotic pressures (339 341 and 263 280 osmol/L )and of pH values(6.5 and 7.0).The results indicated that there wete longer survive(P<0. 01 ) and higher survival index(P<0.01) for diluterswith 6.5 of pH value than those ofdiluters with 7.0 of pH value under the same osmotic pressure.Moreover therewas a longer survive in diluters with higher osmotic pressure under the same pH values.When analyzing thecorrelation between osmotic pressure and pH,We found that the survive of sperm in semen with motility over 0.3was about 30 hours under pH 6.5 and was about 16 hours under pH 7.0 irrespecirive of osmotic pressure levcls.The intact rate of acrosome decreased markedly(P<01 ) under the lower osmotic pressure.