药蒲公英再生体系的建立和优化

Establishment and Optimization of the Regeneration System for Common Dandelion (Taraxacum officinale Weber)

通过愈伤组织诱导和直接不定芽再生途径 ,建立了药蒲公英的快速高效再生系统。叶片外植体在含 0 2mg LIAA和1mg LTDZ的MS培养基中培养 2周后便产生大量的丛生芽 ,在含有 0 5mg L 2 ,4 D和 2mg L 6 BA的MS培养基中培养 30d后 ,形成明显的愈伤组织 ,愈伤组织块在含 1 0mg L 6 BA的MS培养基中成功再生。对 9株再生植株进行RAPD检测表明 ,部分植株在DNA水平上发生了变异。与对照相比 ,再生植株的主要抗氧化成分无明显变化 ,保证了有效成分的稳定。

A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Mult iple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For i ndirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerate d pl antlets were obtained when these calli were cultured on MS medium containing 1. 0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific b ands.