介入化疗对宫颈鳞状细胞癌细胞凋亡和增殖的影响

The Effect of Intra-arterial Influsion Chemotherapy on Apoptosis and Proliferation in Squamous Carcinoma of the Cervix

目的:探讨介入化疗对宫颈鳞癌的细胞凋亡和增殖的影响。方法:选取宫颈鳞癌患者30例,介入化疗前后子宫组织,采用DNA缺口原位末端标记法(TUNEL)检测细胞凋亡,用凋亡指数(AI)表示;免疫组化染色SP法检测Ki67指数表达增殖变化,用LI表示;计算凋亡/增殖比例(AI/LI),并取30例正常宫颈上皮组织作为对照。结果:1)介入化疗后AI明显高于介入化疗前(P<0.05)。2)介入化疗后LI明显低于介入化疗前(P<0.05)。3)介入化疗前AI/LI(0.1293±0.079)低于对照组(0.2033±0.055),介入化疗后AI/LI(0.9956±0.976)高于对照组,介入化疗后AI/LI明显高于介入化疗前,均有统计学意义(P<0.05)。结论:介入化疗能诱导宫颈癌细胞的凋亡,抑制宫颈癌细胞的增殖;AI/LI能表示细胞凋亡和增殖之间平衡。

bjective: To investigate the effect of intra-arterial influsion chemotherapy on apoptosis and proliferation in squamous carcinoma of the cervix. Methods: Cervical tissues in normal cervix group, cervical cancer group before intra-arterial influsion chemotherapy and after intra-arterial influsion chemotherapy were examined and 30 cases were involved in each group. Apoptosis index (AI) was detected by DNA in situ terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). Ki67 labeling index (LI) was detected by SP immunohistochemical technique. The rate of AI/LI was analyzed. Results: 1) Apoptosis index (AI) in cervical cancer group before intra-arterial influsion chemotherapy was significantly lower than that after intra-arterial influsion chemotherapy (P<0.05). 2) Ki67 labeling index (LI) in cervical cancer group before intra-arterial influsion chemotherapy was significantly higher than that after intra-arterial influsion chemotherapy (P<0.05). 3) The rate of AI/LI in cervical cancer group before intra-arterial influsion chemotherapy was lower than that in normal cervix group, the rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy was higher than that in normal cervix group. The rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy was higher than that before intra-arterial influsion chemotherapy (P<0.05). Conclusion: Interventional chemotherapy can induce apoptosis of human cervical cancer cells and inhibit the proliferation. The rate of AI/LI can express the balance of cell apoptosis and proliferation.