化疗药物对T淋巴细胞及其亚群影响的体外探讨

The Study in vitro on Influence of Chemotherapeutics for T Lymphocytes and T Population Subsets

目的:探讨化疗药物对T淋巴细胞及其亚群的影响。方法:取健康成年人外周血T淋巴细胞,采用M TT法观察几种常见化疗药物对T淋巴细胞的杀伤作用,在倒置显微镜下观察化疗后T淋巴细胞的转化能力,应用流式细胞仪比较化疗前后细胞亚群比例的变化。结果:化疗药物在大剂量时对T淋巴细胞的杀伤活力极大,并显著影响淋巴细胞的转化,化疗对T淋巴细胞亚群的杀伤无选择性,不改变细胞亚群的比例(P>0.05)。结论:化疗通过对免疫活性细胞的非选择性杀伤,抑制机体的细胞免疫功能。

Objective: To assess the influence of chemotherapeutics on T cellular immunity and T cell subsets excluding the influence of malignant tumors. Methods: T lymphocytes were acquired from the peripheral blood of a normal adult. The effect of chemotherapeutic drugs on T lymphocytes was measured with MTT. The capability of T lymphoblast transformation was evaluated by observation of morphologic changes of T cells via an upside-down microscope in 48h hours, after T cells incubation with PHA respectively. T cell subsets were measured with flowcytometry before and after the administration of chemotherapeutic drugs. Results: Chemotherapeutic drugs have high cytotoxicity to T lymphocytes at the high level of dosage, but no change the ration of T cell subsets. Conclusions: Cellular immunity can be suppressed by chemotherapeutic drugs, which has a high nonselective cytotoxicity to immune effector cell. The progression and regression of tumors is the main factor influencing the celluar immunity and T cell subsets. Chemotherapy just plays an adjuvant role with a change of the relationship between the body and tumor.