串珠聚糖在肝细胞癌的分布及其免疫组化阳性判断标准的初探

Distribution of perlecan in hepatocellular carcinoma and initial exploration of positive standard of perlcecan for immunohistochemical study

目的 :初步建立串珠聚糖 (PLC)免疫组织化学 (免疫组化 )阳性判断标准 ,探讨PLC在肝细胞癌 (HCC)中的分布特征及其与转移复发的关系。方法 :用免疫组化SP法检测PLC及其配体碱性成纤维细胞生长因子 (bFGF)在HCC中的分布 ,根据PLC分布特征初步建立阳性判断标准 ,分析PLC及bFGF表达与HCC临床病理指标及转移复发的关系。结果 :初步建立了一种PLC阳性判断标准 ;PLC在部分HCC中主要表现为基底膜连续和不连续线形染色 ,部分HCC主要为癌细胞胞质或胞膜棕黄色染色 ;bFGF主要为HCC癌细胞胞质和胞膜棕褐色染色 ;癌组织bFGF表达率及PLC和bFGF共同表达率明显高于癌周肝组织 (分别为P <0 .0 5和P <0 .0 1)。PLC、bFGF表达与HCC临床病理指标及转移复发无关 ;但PLC和bFGF共表达与HCC转移复发有关 (P <0 .0 5 )。结论 :所建立的PLC阳性判断标准具有一定的合理性 ,PLC和bFGF可能参与HCC转移复发和血管生成 ,二者共表达可以预测HCC转移复发。

Objective To establish initially a positive standard of perlcecan (PLC) for immunohistochemical study (IHC), and explore the distribution characteristic of PLC in hepatocellular carcinoma (HCC) and its relationship with metastatic recurrence of HCC.Methods The distributions of PLC, and its ligand, basic fibroblast growth factor (bFGF) in HCC were detected with SP method of IHC, and the positive standard of PLC in HCC was established on the basis of distribution characteristic of PLC, then the relationship between PLC or bFGF expressions and metastatic recurrence of HCC was analyzed. Results An positive standard of perlcecan for IHC was initially established, continuous or discontinuous lineal staining of PLC along basement membrane was observed in partial HCC patients while buffy dyeing in the cytoplasm and membrane of cancer cell in other cases, and bFGF staining was mainly showed as brown granular in the cytoplasm and membrane of cancer cell, both the expression rate of bFGF and coexpression rate of PLC and bFGF in tumor tissue were significantly higher than those in corresponding non-cancerous liver tissue (P<0.05,0.01,respectively).Although expression of PLC or bFGF was independent of the clinical-pathologic indexes and metastatic recurrence of HCC, coexpression of PLC and bFGF was related with metastatic recurrence of HCC(P<0.05). Conclusion The the positive standard of PLC is reasonable to certain extent, and PLC and bFGF may play important roles in the metastatic recurrence and angiogenesis. The coexpression help predict postoperative metastatic recurrence of HCC.