摘要
目的 研究并确定汉坦病毒 (HTNV)病毒吸附蛋白 (VAP)相关表位的序列特征。方法 淘筛噬菌体肽库 ,将阳性克隆携带的外源肽与HTNV囊膜糖蛋白G2做同源性分析。应用IFA及ELISA法鉴定阳性噬菌体的特性。合成短肽 ,结合激光扫描共聚焦显微 (LSCM)技术观察该短肽与宿主细胞膜的结合情况。结果 保守性序列模式PX( 1 2 ) HX( 0 2 ) H与HTNVG2上96 YPWHTAKCHY1 0 5序列相似 ,合成的阳性短肽可以与病毒敏感细胞膜相结合。结论 保守基因序列PX( 1 2 ) HX( 0 2 ) H及与之对应的HTNVG2上96 YPWHTAKCHY1 0 5序列在病毒与宿主细胞结合中可能起作用 。
Objective To identify and characterize the epitope associated with the virus attachment protein(VAP) of hantaan virus. Methods The monoclonal antibody 3G1 was used as the ligand to biopan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid sequences of them were compared with that of hantaan virus G2 in homology. The characteristics of positive phage were studied by IFA and ELISA. A decapeptide combinding to cell membrane was observed under laser scannning confocal microscope (LSCM). Results The conservative motif PX_((1-2))HX_((0-2))H displaying on positive clones shared homologous amino acid sequence with G2 ^(96)YPWHTAKCHY^(105). Conclusion G2 (^(96)YPWHTAKCHY)^(105) might play some roles in virus binding to host cell, and might be a possible key epitope of hantaan virus VAP.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2005年第1期58-60,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金课题 (3 0 174847)
军队"十五"科研规划青年基金课题 (0 1Q115 )