10个加工番茄品种的分子鉴别

Varieties Identification of Processing Tomato Using Molecular Markers

采用 RAPD、RGA和 SRAP分子标记技术对 1 0个加工番茄品种进行了分子鉴别的研究。 1 2个RAPD随机引物扩增后共产生了 2 3个多态性位点 ;2对 RGA引物产生了 1 1个多态性位点 ;1对 SRAP引物产生了 7个多态性位点。分析单引物的品种鉴别能力发现 ,RGA和 SRAP标记具有较高的鉴别效率。通过双引物组合分析发现 ,双引物组合 S391和 em1 + me1或 PK1 + PK2和 em1 + me1可用于 1 0个加工番茄品种的分子鉴别 。

Three kinds of molecular markers, including RAPD, RGA and SRAP were employed to differentiate 10 processing tomato varieties. In this study, 23 polymorphic loci were produced by 12 RAPD primers, 11 polymorphic loci by 2 RGA primer pairs, and 7 polymorphic loci by 1 SRAP primer pair. The informative data by each primer or primer pair were used to identify 10 processing tomato varieties, respectively. It showed that RGA and SRAP marker were more effective. More over, to distinguish the 10 varieties, two-primer-combinations were tested. Primer-combinations, S391 and em1+me1 or PK1+PK2 and em1+me1 could be used to identify the 10 processing tomato varieties and produced specific fingerprint of each varieties.