摘要
目的本研究旨在寻求免疫系统来源的细胞表面存在β-肾上腺素能受体的形态学证据,为进—步研究交感神经系统对免疫系统的调节提供证据。方法采用地高辛未端标记寡核苷酸探针原位杂交技术对淋巴组织的免疫细胞内3种β-肾上腺素能受体mRNA进行了定性检测。结果淋巴组织内存在3种β-肾上腺素能受体的阳性细胞,茁2受体阳性细胞数量较多;在淋巴结内大部分呈弥漫分布,在淋巴组织的淋巴管及小梁、网状支架分布区域有较密集的阳性细胞分布:β3和β1受体阳性细胞数量较少,在淋巴组织内呈弥漫分布,在淋巴组织内分布稀疏。3种β-肾上腺素能受体中β2受体的阳性细胞数量比β1和β3受体的阳性细胞数量多,具有显著的统计学意义(P<0.01),β1和β2受体的阳性细胞数比较无统计学意义(P>0.05)。结论在人类免疫细胞内存在3种β-肾上腺素能受体的mRNA。3种β-肾上腺素能受体亚型在免疫细胞上的分布有差别。
Objective To demonstrate morphologic evidences for the existence of three β-adrenergic receptor (β-AR) subtypes (β1, β2 and β3) mRNA in cells from lymphoid tissue, and to explore the mechanisms of the relationship between sympathetic system and immune system further. Methods The presence of β1, β2 and β3 adrenergic receptors mRNA in the cells of the lymphoid tissue were detected by in situ hybridization(ISH). Results The distribution of β2-AR positive cells was defused except a few positive cells were around the lymphatic channels that more sympathetic nerve fibers innervated. The distribution of β1-AR and β3-AR positive cells were defused. The amount of β2-AR positive ceils was the largest one in three subtypes of β-ARs and showed significant difference(P<0.01). The amount of β1-AR and β3-AR showed no significant difference (P>0.05). Conclusion β-AR mRNA indeed exist in human immune cells. Three β-ARs mRNA have significant difference in human immune cells.
出处
《解剖学研究》
CAS
2005年第1期19-23,共5页
Anatomy Research
基金
山东省自然科学基金资助项目(Y2000C01)
