摘要
随着现代生物医学的发展,人类基因组图谱的完成,应用生物化学和分子生物学技术研究DNA及基因的结构与功能、表达和调控,基因组与生命形式和生物形态进化的关系,使人类的研究进入“后基因组学”时代。本文论述了DNA的探索历程及其在法医学中的应用研究,并对DNA的研究技术基因工程、DNA测序、DNA指纹、PCR和DNA芯片等不同检测方法和实际应用效果、前景加以评定。
出处
《解剖学研究》
CAS
2005年第1期66-68,共3页
Anatomy Research
基金
广东省自然科学基金资助项目(950301)
参考文献24
-
1Portugal FH. Cohen JS. A century of DNA. The Mit Press, 1997. Waston JD, Crick FH. A Structure for Deoxvribose Nucleic Acid. Nature, 1953, 171:737-738.
-
2Collins FS, Galas D. A new five-year plan for the U.S.Hunan Genome Project. Science, 1993,262:43-63.
-
3Jean MC. What if there are only 30000 Human Genes Science, 2001 , 291 : 1255-1257.
-
4Benjamin L. Genes Ⅷ. Oxford University Press, 2000 Bailey JE. Toward a science of metabolic engineering.Science, 1991 , 252: 1668.
-
5Dulbecco R. A turning point in cancer research:sequencing the human genume.Science, 1986,231 : 1055-1056.
-
6Venter JC, The Sequence of the Human Genome.Science, 2001, 291 : 1305-1349.
-
7Venter j, Smith HO, Hood L. A new strategy for genome sequencing. Nature, 1996, 381:363-366.
-
8Jeffreys AJ, Wilson V, Thein SL. Hypervariable minisatellite regions in human DNA. Nature, 1985,314 : 67-73.
-
9Jefferys A J, Brookfileld JF, Semeonoff R. Positive identification of n immigration test-case using human DNA finger prints. Nature, 1985,317:818-819.
-
10Saiki RK, Scharf S, Falloona F, et al. Enzymatic amplification of beta-blobin genomic sequences and restriction analysis for diagnosys of sickle cell anaemia.Science, 1985,230 : 1350-1354.
同被引文献10
-
1Dalbir S, Rajinder P, Chandra P, et al. Linearization of the relationship between serum sodium, potassium concentration, their ratio and time since death in Chandigarh zone of north-west India. Forensic Sci. Int, 2002, 130: 1-7.
-
2Di Nunno N, Costantinides F, Melato M. Determination of the time of death in a homicide-suicide case using flow cytometry. Am J Forensic Med Pathol, 1999,20: 228- 231.
-
3Tomasi De. Improving the technic of the Feulgen stain. Staintech, 1936,11 : 137-144.
-
4Yamada M, Araki T, Yamamoto A. Estimation of residual DNA in Feulgen reaction. A new correction of DNA determination per nucleus. Cell Mol Biol, 1990, 36: 155-161.
-
5Giroud F, Delage B, Monet JD, et al. Methodological considerations on Feulgen-staining applied to cells in primoculture: the model of osteoarthritic synovial cells. Anal Cell Pathol, 1996,11 : 1-12.
-
6Knight B. Changes after death. In Simpson's forensic medicine. 10th ed. London: Edward Arnold, 1991:28- 46.
-
7Gos T, Raszeja SS. Postmortem activity of lactate and malate dehydrogenase in human liver in relation to the time after death. Int J Leg Med, 1993,106:25-29.
-
8Cina SJ. Flow cytometric evaluation of DNA degradation: a predictor of postmortem interval? Am J Forensic Med Pathol, 1994, 15 : 300-302.
-
9Di Nunno NR, Costantinides F, Bernasconi P, et al. Is flow eytometric evaluation of DNA degradation a reliable method to investigate the early postmortem period? Am J Forensic Med Pathol, 1998,19 : 50-53.
-
10陈玉川,成建定.离体人胸骨骨髓DNA降解与腐败尸体死亡时间推断的初步研究[J].法医学杂志,2002,18(3):144-145. 被引量:21
-
1卡洛尔·吉苏克·伊吾恩,柳明(编译).无数生命形态来自几个小小基因[J].创新科技,2008(6):56-57.
-
2现代生物医学进展2011年第11卷(第1~24期)总目次[J].现代生物医学进展,2011,11(S2):5201-5292.
-
3专家认为外星人也许更像终结者阿诺而不是ET[J].信息技术时代,2003(8):68-68.
-
4李建立.蛇岛知多少[J].大自然探索,2006(10):45-49.
-
5吕碧文.SRY——哺乳动物的性别决定基因[J].丽水学院学报,1999,24(5):30-32.
-
6欧阳燕,岳作军,吴黎明,金锋,谭萍萍,刘先蜀.应用生物化学技术进行中华蜜蜂分类的研究[J].云南大学学报(自然科学版),1999,21(S3):231-232. 被引量:2
-
7金振华.生物形态进化和转录调节基因[J].中国科学基金,2000,14(3):143-146.
-
8在那遥远的寒武纪[J].中国科技奖励,2015,0(7):14-15.
-
9吴晓晖,孙璘,徐人尔,陶无凡,邓可京,陈慕云,丁昇,丁一,董永利,杜兴荣,郭增礼,金艳,孔珊珊,雷凯,李荣波,彭超,钱璟熠,时富彪,王光学,王合瑞,徐驰炜,杨东,叶坚,叶知晟,殷琪立,余珊,曾浩,张弛,应蓓蓓,韩珉,庄原,许田.哺乳动物基因功能研究正引发国际生命科学和生物医药产业的新一轮竞争[J].世界科学,2011(1):38-40.
-
10李华,史小平.近交系小鼠遗传监测方法及其研究进展[J].中国实验动物学杂志,1996,6(4):219-221. 被引量:11