氯丙烯、丙烯酰胺和2,5-己二酮对大鼠神经行为改变的比较

Comparative study of neurobehavioral function in rats exposed to allyl chlorice, acrylamide and 2,5-hexanedione

目的:比较氯丙烯(allyl chlorice,AC)、丙烯酰胺(acrylamide,ACR)和2,5-己二酮(2,5-hex- anedione,HD)对大鼠神经行为功能的影响。方法:选用雄性Wistar大鼠,分别给予氯丙烯200 mg/kg体重灌胃,丙烯酰胺40 mg/kg体重腹腔注射,2,5-己二酮400 mg/kg体重腹腔注射,每周3次,定期测定大鼠的体重、热感觉传导、压痛阈值、后肢撑力。结果:2,5-己二酮和氯丙烯使大鼠体重显著降低,而丙烯酰胺对体重的影响不大。氯丙烯中毒后大鼠在热板仪上的保持时间缩短了17%,丙烯酰胺组和2,5-己二酮组的保持时间分别延长了54%和23%。氯丙烯和2,5-己二酮使大鼠压痛阈值明显升高,丙烯酰胺使大鼠的压痛阈值降低。此外,氯丙烯组和丙烯酰胺组后肢撑力指数分别增大了23%和109%,2,5-己二酮组后肢撑力指数变化不大。结论:氯丙烯、丙烯酰胺和2,5-己二酮都可诱导周围神经病,但它们对大鼠神经行为的损伤不同。

Objective: To compare the neurotoxic effects of allyl chlorice (AC), acrylamide (ACR) and 2,5-hexanedione (HD) on neurobehavioral functions in rats. Methods: Male Wistar rats weighting 180-230 g were divided into three groups, and AC was given by gavages 200 mg/kg bw, ACR by ip 40mg/kg bw and HD by ip 400 mg/kg bw, respectively. Neurobehavioral indexes including spontaneous locomotion in an open field, foot splays in hindlimb landing, nervous sensory to pain and heat in hindlimbs, were regularly determined. Results: HD and AC made of rat weight dramatic decrease, but ACR had little effect. The threshold of sensory nerve to heat in AC group decreased by 17%, however, in ACR and HD groups it increased by 54 % and 23% respectively. The threshold of press pain declined in ACR group, but this index of the other two groups rose significantly. The hindlimb landing foot splays of ACR and AC groups increased by 23% and 109% respectively, but it had no significant change in HD group. Conclusion: The neurotoxic effects of AC, ACR and HD on neurobehavioral function in rats are different, even though all of them can cause neuropathy.