非小细胞肺癌中VEGF-C及其受体mRNA的表达与临床意义

Expressions of vascular endothelial growth factor C and its' receptor mRNA in non-small-cell lung cancer

目的:研究肺癌组织及癌旁组织中血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)及其受体mRNA的表达,分析其在肿瘤转移中的作用。方法:采用RT-PCR法分析36例新鲜肺癌组织及癌旁组织标本中VEGF-C/KDR/Flt-4mRNA的表达,并统计分析它与临床病理特点之间的关系。结果:癌旁组织(PT)中VEGF-C mRNA表达与Flt-4mRNA的表达呈显著正相关(r=0.523,P=0.005 5)。肿瘤组织及癌旁组织中单一的VEGF-C、KDR或Flt-4 mRNA表达与肿瘤转移无明显相关;COMVEGF- C-3与淋巴结转移(r=0.580 5,P=0.000 8)及临床病理分期(r=0.505 7,P=0.0064)呈明显正相关。结论: VEGF-C及其受体在肺癌组织及瘤旁组织中高表达,在肿瘤转移尤其是淋巴转移中起重要作用, COMVEGF-C-3是反映患者淋巴转移和预后的良好指标。

Objective: To investigate the expressions of vascular endothelial growth factor-C (VEGF-C) and its' receptor mRNA in human non-small-cell lung cancer tissue and explore their effects on tumor metastasis. Methods: Thirty-six fresh non-small-cell lung cancer tissues and their paratumor tissues were examined by RT-PCR method to detect the expressions of VEGF-C/KDR /Flt-4mRNA. Results: There was a significant positive relation between VEGF-C mRNA and Flt-4 mRNA expressions in paratumour tissues but there was no significant relationships between VEGF-C mRNA, KDR mRNA or Flt-4 mRNA expression and tumor metastasis. COMVEGF-C-3 expression was significantly associated with the grades of tumor pathology (r=0.5057, P=0.0064) and lymph node metastasis(r=0.5805, P=0.000 8). Conclusion: The results suggest that the VEGF-C and its' receptors may play an important role in the lymph node metastasis and COMVEGF-C-3 is a useful biological marker to foresee the lymph node metastasis and prognosis.