雌激素受体亚型在子宫内膜增生中的表达

Expression of estrogen receptor subtypes in endometrial hyperplasia

目的 探讨雌激素受体 (ER)亚型α和βmRNA及蛋白的异常表达在子宫内膜增生的发生发展中的作用。方法 应用半定量逆转录聚合酶链反应 (RT -PCR)和免疫组织化学链霉菌抗生物素蛋白 -过氧化酶连接法 (SP法 ) ,检测 18例正常子宫内膜组织、 4 7例子宫内膜增生组织中ERα和ERβmRNA及蛋白的表达。 结果 ①正常子宫内膜组ERβmRNA的平均表达水平低于子宫内膜增生组 (P <0 0 1) ;②ERαmRNA和蛋白在不典型增生组织中的表达低于单纯型和复合型增生组织 (P <0 0 5 ) ,ERβmRNA和蛋白在这三种类型的子宫内膜增生组织间没有差异。结论 ERβmRNA的异常表达在子宫内膜增生发病中起重要作用 。

Objective To explore the abnormal expression of estrogen receptor(ER)α and β in pathogenesis of endometrial hyperplasia.Methods Using immunohistochemistry and semi-quantitive reverse transcription-polymerase chain reaction(RT-PCR),we examined the expression of ER α、ER β and their mRNA in 18 specimens of normal endometrium and 47 endometrial hyperplasia.Results ① The expression of ER β mRNA in normal edmetrium was lower than in endometrial hyperplasia.② The expression of ER α and its mRNA in atypical hyperplasia was significantly lower than in simple hyperplasia and complex hyperplsia,while the expressions of ER β and its mRNA in three groups above presented no significant difference.Conclusions The abnormal expression of ER β mRNA was closely related to the pathogenesis of endometrial hyperplasia.Decreased expression of ER α and its mRNA might associated with atypical hyperplasia.