耐长春新碱肝癌细胞系HCC-7721/VCR的建立

Establishment of human multidrug-resistant hepatocellular carcinoma cell line HCC-7721/VCR

目的: 培养建立耐长春新碱肝癌细胞系HCC-7721/VCR,并对其生物学特性进行研究.方法: 应用人肝癌细胞系HCC-7721,采用长春新碱大剂量间歇诱导法,建立耐药人肝癌细胞系HCC-7721/VCR.观察该细胞的生长规律;用MTT法鉴定该耐药细胞模型对多种抗癌药物的多药耐药性;以流式细胞技术检测该耐药细胞模型细胞周期分布.结果: 经MTT法鉴定,HCC-7721/VCR细胞较HCC-7721细胞的长春新碱半数致死浓度(IC50)增大了4.15倍,并对多种化疗药物产生耐药性,其耐药性提高了2～4倍不等;耐药细胞倍增时间明显延长,S期细胞减少,而G1、G2期细胞增多.结论: HCC-7721/VCR是一个明确的多药耐药细胞模型,具有耐药细胞的基本生物学特性.

AIM: To establish human multidrug-resistant hepatocellular carcinoma cell line HCC-7721/VCR and to study its biological characteristics.METHODS: Using intermittent administration of high-dose VCR,human hepatocellular carcinoma cell line HCC-7721 was induced to human multidrug-resistant hepatocellular carcinoma cell line HCC-7721/VCR.In vitro drug sensitivity of HCC-7721/VCR and HCC-7721 was determined by MTT assay,and the distribution of their cell cycles was assayed by flow cytometry. RESULTS: ① HCC-7721/VCR was resistant to many chemotherapy agents.The IC 50 value of HCC-7721/VCR to VCR was 4.15 times higher than that of HCC-7721,and its IC 50 to other chemotherapy drugs was 2~4 times higher than that of HCC-7721.② The multiplication time of HCC-7721/VCR prolonged and the number of cells in S-phase decreased while that in G1-phase and G2-phase increased.CONCLUSION: ① We successfully established a multidrug-resistant cell line HCC-7721/VCR,which has the basic characteristics of MDR cells.② Compared with that of its parent cell line HCC-7721,the IC 50 value of HCC-7721/VCR to VCR increases 4.15 times and its distribution of cell cycle changes.