摘要
目的 探讨α- 生育酚琥珀酸酯 (α TOS)诱导人胃癌SGC -790 1细胞凋亡时发挥作用的分子形式。方法 在体外培养的SGC 790 1细胞中 ,分别加入 5、10和 2 0 μg ml的α- TOS ,以 2 0 μg mlα 生育酚 (α- TOH)和1μl/ ml乙醇为对照培养 4 8小时后采用联咪二苯吲哚 (DAPI)染色 ,荧光显微镜下观察细胞形态 ,琼脂糖凝胶电泳检测DNA降解片段 ,并用HPLC法检测细胞内和培养液中α TOS和α- TOH含量。结果 细胞经DAPI染色发现 ,10 μg ml和 2 0 μg mlα TOS处理组细胞核染色体浓集 ,伴有细胞核固缩和核碎片形成。琼脂糖凝胶电泳检测发现 ,上述 2组细胞DNA出现梯形分子条带。在α -TOS处理的细胞内和培养液中HPLC仅检测到α- TOS ,未发现其分解产物α- TOH。结论 α TOS能诱导SGC 790 1细胞凋亡 ,且是以不分解的整体分子形式发挥其诱导凋亡作用 ,与α- TOH的作用无关。
Objective\ In order to explore the effective molecular form of α-tocopherol succinate(α -TOS) in inducing apoptosis of human gastric cancer SGC-7901 c ell. Methods Apoptosis was detected by DAPI staining and DNA ladder, prior to the treatment of cells with α-TOS(5μg/ml,10μg/ml and 20μg/ ml) for 48h, α-tocopherol(α-TOH)( 20μg/ml) and ethanol 1μl/ml as control g roups. HPLC was also used to examine α-TOS and α-TOH in the cells as well as culture medium. Results The observation of DAPI showed that the chromatin in k aryon of SGC-7901 cells in the 10μg/ml and 20μg/ml groups was concentrated wi th karyopycnosis and karyorrhexis, which were not found in other groups. Typical DNA ladders were obvious in the cells post-administration of α-TOS at conce ntrations 10μg/ml and 20μg/ml. It was α-TOS not its hydrolyzed productα-TO H that was screened out by HPLC in cells and culture medium.Conclusion α-TOS was capable of inducting the apoptosis of SGC-7901 cells in its intact form and was independent of α-TOH.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第2期149-151,共3页
Journal of Hygiene Research
基金
黑龙江省科技厅科技攻关项目 (No .GB0 1C1 0 5)