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口蹄疫病毒O/NY00株基因组L片段的克隆及其基因特征 被引量：2

Cloning and Genetic Characterization of L Fragment of the Genome of Foot-and-mouth Disease Virus O/NY00 Strain
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摘要 采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。结果表明:获得的O/NY00株基因组L片段长7805nt,其中包括715at的5’非编码区和6999nt的多聚蛋白编码区。将O/NY00株与其它参考毒株进行序列比较,结果显示:O/NY00株与O/SKR/2000、O/UKG/3/2001遗传关系最近,而与其它毒株遗传关系较远,属于O型口蹄疫ME-SA拓扑型PanAsia株的成员。与Cathay拓扑型的代表毒株O/TAW/97比较,两者在主要抗原位点1和位点3呈现出明显的以拓扑型为特征的氨基酸差别,提示两拓扑型毒株之间有较高的抗原差异。 The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR,and sequenced.The results showed that L genomic fragment of O/NY00 isolate is 7 731nt in length including partial 5' UTR and complete polyprotein coding region with 715nt and 6 999nt respectively .Comparison among O/NY00 and other reference isolates indicated that O/NY00,O/SKR/2000 and O/UKG/ 3/2001 may come from the same ancestor, and O/NY00 should be classified into PanAsia strain of FMDV serotype O ME - SA topotype. Furthermore, comparison of deduced amino acid residues at the antigenic sites with O/TAW/97,a typical isolate of Cathay topotype,showed there are obviouslly topotypical amino acid differences at Site 1 and Site 3, thus, suggesting the less antigenic relationships between these two topotype isolates.
作者 郑敏 金宁一 张洪勇 刘棋 韦婷 郭建刚 李华明 尹革芬 鲁会军
机构地区 军事医学科学院军事兽医研究所 广西区兽医防疫检疫站
出处 《病毒学报》 CAS CSCD 北大核心 2005年第2期124-130,共7页 Chinese Journal of Virology
基金 国家863计划资助项目(2001AA213071)吉林省科技厅重点项目(20040202-1)
关键词 毒株 口蹄疫病毒 遗传关系 基因组 非编码区 抗原位点 序列比较 基因特征 RT—PCR方法 SA foot-and-mouth disease virus(FMDV) O/NY00 isolate genome L fragment molecular cloning sequence analysis
分类号 Q422 [生物学—神经生物学] S852 [农业科学—基础兽医学]
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参考文献10

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共引文献49

同被引文献20

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病毒学报
2005年 第2期

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