摘要
目的 :研究游泳训练后大鼠腓肠肌二价金属离子转运体 1 (divalentmetaltransporter1 ,DMT1 )、转铁蛋白受体 (transferrinreceptor,TfR)及膜铁转运蛋白 1 (ferroportin1 ,FPN1 )表达的变化 ,探究运动对腓肠肌铁代谢的影响机制。方法 :4 0只雌性SD大鼠随机分为对照组和游泳训练组 ,分别于游泳训练 5周及 1 0周后取材 ,测定血清铁状态、腓肠肌非血红素铁含量 ,采用WesternBlot方法检测腓肠肌铁代谢相关蛋白表达的变化。结果 :(1 )游泳训练 5周及 1 0周组大鼠血清铁、Hb及RBC均较对照组显著升高 ,游泳运动 1 0周组大鼠腓肠肌非血红素铁含量显著增加 (P <0 . 0 1 ) ;(2 )游泳训练组大鼠腓肠肌DMT1 (IRE)和TfR表达增加 ,FPN1表达减少 (P <0 . 0 5 ) ,而DMT1 (non -IRE)表达无明显变化。结论 :游泳训练可以增加腓肠肌非血红素铁含量 ,促进机体铁动员。这可能与腓肠肌细胞膜上向胞内转运铁的TfR和DMT1(IRE)蛋白表达增加 ,而向胞外转运铁的FPN1表达下降有关。
Objective To investigate the effects of swim training on the expression of divalent metal transporter 1(DMT1), transferrin receptor(TfR) and ferroportin 1(FRN1) in rats' gastrocnemius. Methods 40 female SD rats were randomly divided into two groups: control group(CG)and exercise group(EG), and were sampled after 5 and 10-week swimming. Determined serum iron status and non-heam iron of gastrocnemius. Observed the expression of TfR, DMT1 and FPN1 by Western Blot. Results (1)The serum iron, Hb and RBC were significantly higher than those in control group. The gastrocnemius' non-heam iron of exercise group were significantly increased after 10-week training(P<0.01). (2) The expression of DMT1 (IRE) and TfR of exercise group was increased, but FPN1 expression was decreased, and DMT1 (non-IRE) had no change. Conclusion Suitable training could improve body iron homeostasis, increase gastrocnemius' non-heam iron. The mechanism of changes were that the expression of DMT1 (IRE) and TfR were increased but that of FPN1 was decreased.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2005年第2期147-151,共5页
Chinese Journal of Sports Medicine
基金
河北省自然基金项目 (30 31 5 8)
河北省教育厅博士科研基金 (B2 0 0 31 2 4 )
河北师范大学博士科研基金 (L2 0 0 3B1 5 )资助。
