运动性中枢疲劳时大鼠脑乳酸和糖原含量的变化

Changes of Glycogen and Lactate in Cortex of Rats after Exercise-Induced Central System Fatigue

目的 :探讨运动性中枢疲劳时大鼠脑乳酸和糖原含量的变化。方法 :运用Bedford递增负荷运动方案建立运动性中枢疲劳模型。将Wistar大鼠随机分为 3组 :对照组、一次性力竭组和过度训练组。检测大脑皮层乳酸和糖原含量。结果 :与对照组相比 ,一次性力竭组和过度训练组大鼠体重增加缓慢 ,7天分别增加 1 3 . 33± 7 . 5 3g和 1 0 . 0 0± 6 . 32g ;血尿素氮分别为 4 . 78± 1 . 99mmol/L和8 . 4 8± 0 . 92mmol/L ,过度训练组较对照组和一次性力竭组升高明显 (P <0 . 0 5 ) ;各组血红蛋白含量无明显变化。一次性力竭组和过度训练组大鼠大脑皮层 5 -HT含量分别为 91 7 . 4 4± 1 6 0 . 73ng/ml·g和 730 . 89± 392 . 2 5ng/ml·g ,但无显著差异。过度训练组DA含量为 30 9 . 0 4± 6 3 . 6 8ng/ml·g ,DA/ 5-HT为 0 . 4 2± 0 . 2 5 ,与对照组相比显著降低 ;SEP潜伏期延长 ,P1 :2 98± 0 5 5ms ,N1 :4 2 3±0 35ms ,P2 :5 2 8± 0 . 6 0ms。一次性力竭组脑 5 -HT含量、DA含量DA/ 5 -HT及SEP潜伏期与对照组相比均无显著变化。一次性力竭组大脑皮层乳酸含量 (1 . 1 2mg/ 1 0 0g)和过度训练组 (1 . 4 6mg/1 0 0g)明显高于对照组 (0 . 92mg/ 1 0 0g)。各组大脑皮层糖原含量变化无显著性?

Objective In order to investigate the changes of glycogen and lactate in cortex after exercise-induced central system fatigue. Methods Central fatigue model was established according to Bedford weight/maximal oxygen uptake equation. The rats were divided into three groups: control group (C), sedentary-exhausted group (SE), and overtraining group (O). Results After 1 week exercise, compared with control group, the weight of rats in SE and O group increased slowly. BUN of SE and O group increased significantly to 4.78±1.99 mmol/L and 8.48 ±0.92 mmol/L respectively,but Hb showed no changes. Compared with C group, both DA and DA/5-HT of O group decreased dynamical which were 309.04±63.68 ng/ml·g and 0.42±0.25 respectively. The latency of SEP in O group distinctly delayed, P1: 2.98±0.55ms, N1: 4.23±0.35ms, P2: 5.28±0.60ms. Regarding to 5-HT, no significant difference was found among all groups. Cortex lactate concentration of O (1.46mg/100g )and SE(1.12 mg/100g) group were significantly higher than control group(0.92 mg/100g). There was a tendency of glycogen decrease but had no significant difference among all groups. Conclusion The results suggested that lactate and glycogen might have close relationship with central fatigue and were the possible reasons to cause central fatigue.