摘要
目的 :探讨运动训练中补充褪黑激素 (Melatonin ,MT)对机体抗氧化系统的影响 ,为选用MT作为抗氧化剂或体能恢复剂提供实验依据。方法 :以雄性昆明种小鼠为实验对象 ,采用递增负荷耐力训练及一次性力竭运动建立模型。分别测定肾脏还原型谷胱甘肽 (ReducedGlutathione ,GSH)含量 ,肝脏、骨骼肌与肾脏的超氧化物歧化酶 (SuperoxideDismutase ,SOD)、过氧化物酶 (Peroxi dase ,POD)、过氧化氢酶 (Catalase ,CAT)的活性、总抗氧化能力 (TotalAnti -oxidationCapability ,T -AOC)与丙二醛 (Malondialdehyde ,MDA)含量。结果 :安静状态下 ,服用MT可显著提高肾脏GSH含量与SOD活性、肾与肝T -AOC及肝POD与CAT活性 (P <0 . 0 5~ 0 . 0 1 ) ,显著降低骨骼肌、肝与肾MDA含量 (P <0. 0 1 ) ,但明显抑制骨骼肌CAT活性 (P <0 . 0 1 ) ,对其余指标无显著影响。力竭运动后即刻 ,补充MT组大鼠骨骼肌、肾与肝T -AOC、肾与肝POD活性、骨骼肌MDA含量与肾GSH含量均显著高于对照组 (P <0 . 0 5~ 0 . 0 1 ) ,而肾与肝SOD活性、肾CAT活性与MDA含量均显著低于对照组 (P <0 . 0 5~ 0 . 0 1 ) ,两组间其余指标无显著性差异。 2 4h恢复后 ,服用MT组大鼠骨骼肌、肾与肝SOD与POD活性、骨骼肌与肾CAT活性、肾T
Objective The experiment is to investigate the effects of melatonin(MT) on endurance-trained mice' anti-oxidation system in liver, skeletal muscle and kidney which may support the usage of MT as antioxidant to improve performance. Methods Male Kunming mice were used to set up animal model by endurance-training and exhaustive exercise. The activity of superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) and the content of total anti-oxidation capability(T-AOC), malondialdehyde(MDA) in liver,skeletal muscle and kidney and glutathione (GSH) in kidney were measured. Results In sedentary condition, MT can significantly enhance the activity of SOD and the content of GSH in kidney, the content of T-AOC in kidney and liver, the activity of POD and CAT in liver(P<0.05~0.01) and significantly decrease the content of MDA in liver, skeletal muscle and kidney(P<0.01), but significantly restain CAT activity in skeletal muscle (P<0.01). Shortly after exhaustive training, in MT group, T-AOC content in skeletal muscle, kidney and liver, the activity of POD in kidney and liver, MDA content in skeletal muscle and GSH content in kidney were significantly higher (P<0.05~0.01), but the activity of SOD in liver and kidney, CAT activity in kidney, MDA content were significantly lower than that in controls (P<0.05~0.01). After 24 hours restoration, in MT group, the activity of SOD and POD in skeletal muscle, kidney and liver, CAT activity in liver and kidney, T-AOC and GSH content in kidney were significantly higher(P<0.05~0.01), but the MDA content in skeletal muscle and liver were significantly lower than that in controls (P<0.01). Conclusions The results suggested that MT could prevent the anti-oxidation system from exercise-induced damage, which might be one of mechanisms that MT could prevent exercise-induced fatigue.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2005年第2期210-213,共4页
Chinese Journal of Sports Medicine