IL-24基因对大鼠胶质瘤细胞生长状况的影响

Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo

目的 探讨IL 2 4基因对C6大鼠胶质瘤细胞生长状况的影响。方法 应用逆转录病毒载体 ,将IL 2 4基因导入C6细胞 ,经G4 18筛选后获得表达IL 2 4分子的阳性细胞克隆C6 /IL 2 4 ;用RT PCR方法检测目的基因表达 ;四甲基偶氮唑蓝 (MTT)法检测细胞体外增殖状况 ,流式细胞技术检测细胞的增殖活性 ,并制作荷瘤动物模型 ,观察C6 /IL 2 4和C6细胞的体内致瘤性。结果 RT PCR检测表明 ,外源IL 2 4基因于mRNA水平在C6 /IL 2 4细胞已获得稳定表达。C6 /IL 2 4细胞系的体外增殖性较亲代C6细胞明显下降 ,流式细胞术检测其细胞增殖指数 (PI)为 (2 9.71± 0 .89) %。 9只接种C6 /IL 2 4细胞的实验组大鼠中 ,6只颅内成瘤 ,肿瘤体积为 (14 .0 8± 9.81)mm3 ,明显小于接种C6细胞大鼠的肿瘤体积 (P <0 .0 5 )。结论 外源性IL 2 4基因可部分抑制胶质瘤细胞异常增殖的肿瘤特性。

Objective To investigate the effect of IL 24 expression on the growth of glioma cells. Methods The IL 24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL 24 in C6/IL 24 glioma cells was confirmed by RT PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor. Results It was confirmed by RT PCR that the exogenous IL 24 gene expressed in C6/IL 24 cell. The C6/IL 24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell. Conclusion IL 24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.