革兰阴性杆菌感染患者血浆内毒素测定的临床意义

Plasma Endotoxin Detection for Patient with Gram Negative Bacillus Infection: Its Clinical Significance

目的探讨革兰阴性杆菌感染患者和正常健康人群血浆内毒素水平及临床意义。方法分别用定量动态 比浊法和鲎试剂定性凝胶法检测革兰阴性菌感染患者和正常健康对照血浆内毒素含量。结果 正常对照组血浆 内毒素定量(3.30±k1.73)pg／ml,定性全部为阴性;革兰阴性菌感染组内毒素(945士2 244.43)pg／ml,定性阳性 率为68.19％;经SPSS统计软件t检验分析,对照组与革兰阴性菌感染组内毒素平均值差异显著(t=2.608, P=0.025),相关性分析表明定量动态比浊法和鲎试剂定性凝胶法在0.01水平相关。结论检测血浆内毒素含 量有助于早期判断感染的细菌种类及是否存在内毒素血症,有利于临床合理应用抗菌药物和及时对症处理。

OBJECTIVE To study the clinical significance of plasma endotoxin detection for patient with Gram negative bacillus infection. METHODS Plasma endotoxin of patient and healthy people was detected by turbidimet-ric assay and gel-clot assay with Limulus reagents. RESULTS Mean plasma entoxin of healthy people was (3. 30± 1. 73) pg/ml and gel-clot assay was all negative. In Gram negative bacillus infection group, the result was (945± 2 244.43) pg/ml and the positive rate of gel-clot assay was 68. 19%. ?Test analysis indicated the mean plasma endotoxin of two groups had significant difference( t =2. 608,P=0. 025). Relativity analysis indicated there was correlation between turbidimetric assay and gel-clot assay on 0. 01 level. CONCLUSIONS Plasma endotoxin detection is helping to estimate the endotoxemia and what kind of bacteria be infected. It is also of significance to select antibacterials rationally and handle syndrome on time.