饲喂重组鸡白细胞介素18蛋白增加肉仔鸡体重的初步研究

Efficacy of Recombinant Chicken Interleukine 18 Protein on Weight Gain for Broiler Chickens

经口服途径给肉仔鸡饲喂重组鸡白细胞介素 1 8(ChIL -1 8)蛋白 ,观察其在正常饲养条件下和人工感染鸡传染性支气管炎病毒 (IBV)时对肉仔鸡的影响。将 84只肉仔鸡随机分为 2组 :正常称重组和IBV接种组。 2组肉仔鸡再随机各分为 3个亚组 ,分别每隔 5d各口服 1次PBS、细菌蛋白和重组ChIL -1 8蛋白。正常称重组每亚组 8只 ,每次口服前称重。IBV接种试验组每亚组 2 0只 ,在 30日龄时滴鼻接种IBVM41株并每隔 5d称重 1次。各亚组试验鸡分别在正压隔离器中饲养。结果表明 ,饲喂重组ChIL- 1 8蛋白的试验亚组肉仔鸡体重总增重量明显高于饲喂PBS和细菌蛋白的对照亚组 ;IBV接种试验组肉仔鸡在人工感染IBVM41株后 ,饲喂重组ChIL 1 8亚组的发病率和死亡率明显低于饲喂PBS和细菌蛋白亚组 ,而且后 2个亚组肉仔鸡临床症状也较饲喂重组ChIL- 1 8试验亚组明显。结果初步显示 ,饲喂重组ChIL -1 8融合蛋白能够增加肉仔鸡体重并可增强肉仔鸡对IBV感染的抵抗能力。

Eighty broiler chickens were used to evaluate the efficacy of recombinant ChIL 18 protein in vivo.Broilers were orally administrated with recombinant chicken Interleukine 18 (ChIL 18) protein expressed in E.coli,PBS and bacterial protein,respectively.Efficacy of the recombinant protein on broiler birds that reared under normal condition and IBV infection condition was investigated.As a result,the total weight gain of the birds administrated with ChIL 18 is obviously higher than those of administrating with PBS or bacterial protein.Furthermore,IBV infection trial suggested that orally administration with recombinant ChIL 18 to the IBV infected birds could enhance their growth performance and less susceptibility to IBV infection compared to non treated infected birds.The result study showed that recombinant chIL 18 protein can be absorbed in the digestive tract of the chickens and plays an important role in weight gain and resistance against IBV.