摘要
目的 探讨食管上皮癌变过程中细胞周期调控因子cyclin E、CDK2和p21WAF1的表达状况及其意义。方法 应用免疫组化SP法和原位杂交方法分别检测48例食管癌组织、31例非典型增生组织和17例正常食管粘膜中cyclin E、CDK2和p21WAF1蛋白及mRNA表达。应用半定量RT PCR和Western blot检测22例新鲜食管癌及相应癌旁组织的mRNA和蛋白表达。结果 从食管正常粘膜、非典型增生组织到癌组织,cyclin E和CDK2蛋白和mRNA阳性表达率逐渐上升,差异具有统计学意义(P<0.01或P<0.05)。食管癌组织中cyclin E、CDK2和p21WAF1蛋白及mRNA高表达,与癌旁组织或切缘正常食管粘膜有显著性差异(P<0.01)。cyclin E、CDK2和p21WAF1 基因表达显著正相关(P<0.01 或P<0.05)。结论 食管上皮癌变过程中,细胞周期相关基因cyclin E和CDK2表达逐渐增强。cyclin E基因表达异常是食管癌变过程中的早期事件。p21WAF1 基因在食管癌中高表达,可能与细胞周期调控的反馈机制有关。
Objective To investigate the effects of the gene e xpressions of cyclin E, CDK 2 and p21 WAF1 in esophageal carcinogenesi s.Methods Immunohistochemical and in situ hybridization metho ds were applied to detect the expression of cyclin E,CDK 2 and p21 WAF1 proteins and mRNAs in 48 cases of esophageal carcinoma,31 cases of dysplasia and 17 cases of normal epithelium. Twenty two cases of fresh esophageal carcinom a tissue and corresponding adjacent noncancerous tissues were examined by wester n blot and semiquantitative RT-PCR for the protein and mRNA expressions for cyc lin E,CDK 2 and p21 WAF1.Results The positive rates of cyclin E and CDK 2 proteins and mRNAs were increased from normal epithelium to dysplasia and carcinoma tissu es gradually(P<0.01 or P< 0.05). Cyclin E,CDK 2 and p21 WAF 1 overexpressions were frequently found in esophageal carcinoma and there was very significant statistical difference between carcinoma and corresponding adja cent noncancerous tissue or normal epithelium (P<0.01). There was significan t positive correlation among cyclin E,CDK 2 and p21 WAF1gene expressio ns(P<0.01 or P< 0.05).Conclusion Elevated levels of cyclin E and CDK 2 are obser ved gradually during esophageal carcinogenesis. Abnormal expression of cyclin E is an early event in esophageal carcinogenesis. The overexpression of p21 WAF 1gene in esophageal carcinoma is probably due to the feedback control loop in cell cycle.
出处
《肿瘤》
CAS
CSCD
北大核心
2005年第2期158-162,共5页
Tumor
基金
河北省自然科学基金资助项目(编号:301354)
