摘要
对分布于中国的飞蝗Locusta migratoria Linnaeus 3亚种的线粒体12S rDNA 487 (bp)和 ND5 (451 bp) 基因的部分序列进行测定,与已知全线粒体基因组全序列的非洲飞蝗亚种进行序列比较,并在此基础上对ND5基因,以斑腿蝗科瘤喉蝗Parapodismamikado 为外群,重建 MP 树。基于序列长度的限制,将两基因联合分析重建了 1 棵最简约无根树。在对准的 4 个亚种共有的 481 bp 的 12S rDNA 和 451 bp 的 ND5 片断序列比较中,AT 含量明显高于 GC 含量。由序列差异来看,亚洲飞蝗和东亚飞蝗序列相似度高,非洲飞蝗和西藏飞蝗相似度高,ND5 基因的 MP 树和联合树也支持这个结论。由于大陆漂移和青藏高原隆起的特殊地质事件,正好解释了非洲飞蝗和西藏飞蝗的相似性,支持西藏飞蝗为单独 1 亚种,与非洲飞蝗为同一起源地,与其它 2 亚种相区别。
The partial 12S rDNA(487 bp)and ND5(451 bp)gene fragments of the three subspecies of Locusta migratoria Linn in China were sequenced. The sequences were aligned to the corresponding segment of L. m. migratorioides and compared to each other. The MP topology was reconstructed from the partial sequence of ND5 with outgroup Parapodisma mikado and an unrooted MP tree was reconstructed based on the combined sequences of 12S rDNA and ND5 from four subspecies. Those sequences all have the character of high AT content, which is consistent with results in other arthropods. Both sequences comparison and MP topology support the similarity of L. m. tibetensis and L. m. migratorioides, and given the geography distribution of them, we conclude the subspecies status of L. m. tibetensis and consider the similarity of L. m. tibetensis and L. m. migratorioides as the result of geological events: continent float and ascent of Qinghai-Xizang Plateau. L. m. tibetensis and L. m. migratorioides have the same origin area and differentiate them from the other two subspecies.
基金
国家自然科学基金项目(30070114)
陕西师范大学 2002 年研究生培养创新基金
